Protein Info for H281DRAFT_04077 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Isoleucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 945 TIGR00392: isoleucine--tRNA ligase" amino acids 23 to 859 (837 residues), 863.7 bits, see alignment E=7.9e-264 PF00133: tRNA-synt_1" amino acids 35 to 660 (626 residues), 450.6 bits, see alignment E=1.3e-138 PF09334: tRNA-synt_1g" amino acids 66 to 206 (141 residues), 46.8 bits, see alignment E=4.8e-16 PF08264: Anticodon_1" amino acids 705 to 859 (155 residues), 86.9 bits, see alignment E=3.2e-28 PF06827: zf-FPG_IleRS" amino acids 907 to 934 (28 residues), 38.5 bits, see alignment (E = 2e-13)

Best Hits

Swiss-Prot: 92% identical to SYI_PARP8: Isoleucine--tRNA ligase (ileS) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 87% identity to bmu:Bmul_0783)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MD13 at UniProt or InterPro

Protein Sequence (945 amino acids)

>H281DRAFT_04077 Isoleucyl-tRNA synthetase (Paraburkholderia bryophila 376MFSha3.1)
MSNKKADSKPPSRYPVNLLDTPFPMRGDLPKREPQWVKEWQERKVYEKIRAASKGRKKFI
LHDGPPYANGDIHLGHAVNKILKDMIVKARNMAGFDAVYVPGWDCHGMPIEIQIEKQFGK
SLPAAEVMQKARAYATEQIEKQKVGFRRLGVLGEWDNPYKTMNFANEAGEIRALAKIMEK
GYVFRGLKPVNWCFDCGSALAEAEVEYKDKTDPTIDVLFSFAEPEKTAQAFGLAALPRNE
GGIVIWTTTPWTIPANQALNLHPEIVYALVDTPRGLLILAEERVEACLNAYGLEGSVVAT
TPGAKLVNLRFNHPLASAHPGYKRTAPVYLGDYVTTETGTGVVHSSPAYGVEDFVSCKAH
GMADSDIINPVMGDGRYIESLALFGGLSIWAANPQIVEALQGAGSLLRTEKYTHSYMHCW
RHKTPIIYRATSQWFAGMDVKPNDTDKTLRETALEGIENTAFYPSWGKQRLFSMIANRPD
WTLSRQRQWGVPMAFFVHKETGELHPRTLELLEEVAKRVEEAGIEAWQTLDPRELIGDDA
NMYEKNRDTLDVWFDSGTTHWHVLRGSHKDELQFPADLYLEGSDQHRGWFHSSLLTASML
DGRPPYNALLTHGFTVDGEGRKMSKSLGNGIDPHEVANRLGAEIIRLWIASTDYSGELAI
SEEILKRVTEGYRRIRNTLRFLLANLSDFDFEKNARPVGDWLEIDRYAVALSANLQNDIL
SHYDKYEFHPVVAKLQTFCSEDLGGFYLDVLKDRLYTTAADSVARRSAQTALYHIAQGLL
RLMAPFLSFTAEEAWKVFQPNSETIFTETYHAFPAVPDAGALLDKWTLLRAARGDVTKAL
EEARVANLIGSSLQAEVEIRASGARYDALKSLGDDLKFVLITSAASVVKVESEAEEGVEV
ITSKYLKCERCWHYRADVGADAEHPTLCGRCVSNLFGNGEARSAA