Protein Info for H281DRAFT_04004 in Paraburkholderia bryophila 376MFSha3.1

Annotation: (p)ppGpp synthetase I, SpoT/RelA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 786 TIGR00691: RelA/SpoT family protein" amino acids 76 to 761 (686 residues), 742.9 bits, see alignment E=1.8e-227 PF13328: HD_4" amino acids 76 to 225 (150 residues), 178.3 bits, see alignment E=2.8e-56 PF01966: HD" amino acids 95 to 194 (100 residues), 43 bits, see alignment E=1.6e-14 PF04607: RelA_SpoT" amino acids 285 to 394 (110 residues), 131.6 bits, see alignment E=4.6e-42 PF02824: TGS" amino acids 438 to 497 (60 residues), 69.5 bits, see alignment 5.9e-23 PF19296: RelA_AH_RIS" amino acids 511 to 592 (82 residues), 29.2 bits, see alignment E=2.9e-10 PF13291: ACT_4" amino acids 685 to 762 (78 residues), 54.1 bits, see alignment E=5.9e-18

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 99% identity to bug:BC1001_2709)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MEW4 at UniProt or InterPro

Protein Sequence (786 amino acids)

>H281DRAFT_04004 (p)ppGpp synthetase I, SpoT/RelA (Paraburkholderia bryophila 376MFSha3.1)
MSTTPPTATEAEHDADSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTSA
LSGYLQPEEIKDVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWNLDGQAIMAAL
LHDVMEDQGVTKAELAERFGAKVAELVDGLSKLDKMEFRNREEAQAENFRKMLLAMARDV
RVILVKLADRLHNMRTLGAVPPEKRRRVARETLDIYAPIAHRLGLNNTYRELQDLSFANF
NPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKIDAEVTGREKTIFSIYKKMRDK
QLSFSQVLDVYGFRVVVDSALECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTL
VGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGSADLNDVQKRAHQWLKSLLDIQSEAG
DSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNEL
LPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLV
DQALKGYGLALSDVTPEAWEKLVQWTGNKNGQEIFADIGLGRRVAAVMAKRIEVLMSGRD
ADDDGSRSESGSTPHAPPVVITGTEGMSVQLSACCRPIPGDDIMGYIGIGLGMAIHTTDC
RVAQRIHRRDPGRWIDVAWAPQPGRLFDVAIKVLVKNTKGVFARVAADITSADANIVHIA
MDEDLSQESTILRFVIQVSDRVHLANVMRRVRTNLDVMRIARERPSDDGHHRHDGGMRID
RERADY