Protein Info for H281DRAFT_03942 in Paraburkholderia bryophila 376MFSha3.1

Annotation: formate dehydrogenase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 983 PF13510: Fer2_4" amino acids 50 to 124 (75 residues), 88.2 bits, see alignment 1e-28 PF10588: NADH-G_4Fe-4S_3" amino acids 131 to 170 (40 residues), 59.3 bits, see alignment (E = 7.6e-20) PF12838: Fer4_7" amino acids 196 to 253 (58 residues), 31.3 bits, see alignment 8.3e-11 PF00037: Fer4" amino acids 236 to 253 (18 residues), 24.9 bits, see alignment (E = 4.7e-09) PF04879: Molybdop_Fe4S4" amino acids 268 to 319 (52 residues), 58 bits, see alignment 2.5e-19 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 272 to 947 (676 residues), 934.3 bits, see alignment E=1.7e-285 PF00384: Molybdopterin" amino acids 324 to 741 (418 residues), 243.8 bits, see alignment E=9.7e-76 PF01568: Molydop_binding" amino acids 839 to 944 (106 residues), 106.1 bits, see alignment E=3.5e-34

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 94% identity to bpy:Bphyt_3023)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MDB3 at UniProt or InterPro

Protein Sequence (983 amino acids)

>H281DRAFT_03942 formate dehydrogenase alpha subunit (Paraburkholderia bryophila 376MFSha3.1)
MSDMLNTPAGGCGSGNCACKSQAQAQSRTPRSYFDDTDYGTPERHADIDVTLEIDGQAVT
VPAGTSVMRAAIEAGVNVPKLCATDSLEPFGSCRLCLVEIEGKRGYPASCTTPVEAGMKV
RTQSDRLQSLRRNVMELYISDHPLDCLTCPANGDCELQDMAGVTGLREVRYGIDGANHLQ
DKKDESNPYFTYDPAKCIVCNRCVRACEETQGTFALTIAGRGFESRVAASENQPFMESEC
VSCGACVSACPTATLQEKTVVMLGQAEHSVVTTCAYCGVGCSFKAEMKGNQVVRMVPFKN
GQANEGHACVKGRFAWGYATHKDRITKPMIRAKITDPWREVSWEEAINYAASEFRRIQAK
HGRDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKTTLGESAGT
QTFASVDKADVIMVIGANPTDGHPVFGSRLKRRVREGAKLIVVDPRRIDIVDTPHVKASH
HLQLRPGTNVAIVNALAHVIVTEGLLNEAFISERCEGRAFDQWRDFVALPENAPEATEAV
TGVPAKDVREAARIYATGGNAAIYYGLGVTEHAQGSTMVMGIANLAMATGNIGREGVGVN
PLRGQNNVQGSCDMGSFPHELPGYRHISDTIVRSQFEDAWNVTLQTEPGLRIPNMFEAAL
DGSFMGLYCQGEDIVQSDPNTHHVAAALSSMECIVVQDIFLNETAKYAHVLLPGSTFLEK
DGTFTNAERRISRVRKVMPPVPGYADWEVTLLLSRALGYEMDYAHPSEIMDEIARLTPTF
HGVSYKKIDELGSIQWPCNEEAPDGTPTMHIDTFVRGKGKFVITKFIASPEKVTRKFPLL
LTTGRILSQYNVGAQTRRTENSRWHDEDRLELHPHDAEERGIKTGDWVGIESRAGQTVLR
AKVTERMQPGVVYTTFHFPESGANVITTDSSDWATNCPEYKVTAVQVMPVEQPSQWQKEY
SRFNAEQLELLKQREMANATAGK