Protein Info for H281DRAFT_03941 in Paraburkholderia bryophila 376MFSha3.1

Annotation: formate dehydrogenase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 521 transmembrane" amino acids 199 to 221 (23 residues), see Phobius details amino acids 305 to 325 (21 residues), see Phobius details PF01512: Complex1_51K" amino acids 146 to 315 (170 residues), 153.8 bits, see alignment E=3.6e-49 PF10589: NADH_4Fe-4S" amino acids 427 to 507 (81 residues), 95.8 bits, see alignment E=1.1e-31

Best Hits

KEGG orthology group: K00124, formate dehydrogenase, beta subunit [EC: 1.2.1.2] (inferred from 96% identity to bgf:BC1003_2620)

Predicted SEED Role

"NAD-dependent formate dehydrogenase beta subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MCX3 at UniProt or InterPro

Protein Sequence (521 amino acids)

>H281DRAFT_03941 formate dehydrogenase beta subunit (Paraburkholderia bryophila 376MFSha3.1)
MTRIYVPRDSSALALGADALADAIKAEAARRGIAIDLIRNGSRGLLWLEPLVEVQTAEGR
VGYANVEESDVGALFDAGFVDGGEHARRVGVVDEIPYLKKQQRLTFARIGITDPLSTDDY
VAHGGLEGLRQALQTDGDAACEALIESGLRGRGGAAFPAGIKWRTVRAAKAAQKYIVCNA
DEGDSGTFSDRLVMESDPYVLIEGMIIAGVVTGATAGYIYVRSEYPHSIATLEAAIAKAR
AAGWLGDSVLGSAHRFELFVAKGAGSYVCGEETALLESLEGKRGIVRAKPPVPALVGLYG
QPTVINNVITLATVPIIFARGAAFYKDFGMGRSRGTLPFQLAGNVKQGGLVELAFGVTLR
ELIHNYGGGTASGRPARAVQVGGPLGTYLPESQWDIPMDYEAYAAVGAVVGHGGLVVHDD
TSNLAELAQYAMHFCALESCGKCTPCRIGSTRGVEVIARIRNGDTSTRQVQLLRDLCDTM
VSGSLCAMGGMTPYPVLSALDHFPEDFGLADARADAPKAAA