Protein Info for H281DRAFT_03846 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 3-phenylpropionate/trans-cinnamate dioxygenase ferredoxin reductase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 419 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 4 to 304 (301 residues), 221.6 bits, see alignment E=6.1e-69 PF00070: Pyr_redox" amino acids 146 to 225 (80 residues), 64.7 bits, see alignment E=3.6e-21 PF13450: NAD_binding_8" amino acids 149 to 186 (38 residues), 23.7 bits, see alignment 2e-08 PF14759: Reductase_C" amino acids 323 to 406 (84 residues), 106.3 bits, see alignment E=3.8e-34

Best Hits

KEGG orthology group: K00529, ferredoxin--NAD+ reductase [EC: 1.18.1.3] (inferred from 97% identity to bug:BC1001_4036)

Predicted SEED Role

"Ferredoxin reductase" in subsystem Anaerobic respiratory reductases

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.1.3

Use Curated BLAST to search for 1.18.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (419 amino acids)

>H281DRAFT_03846 3-phenylpropionate/trans-cinnamate dioxygenase ferredoxin reductase subunit (Paraburkholderia bryophila 376MFSha3.1)
MKPRLVIVGASYAGLQLAASARESGHDGDIVLIGDEPHAPYQRPPLSKGFLTGSFAEERL
PLRSPAFYGEMRMQWMPSTRALRIDRERKQIELHDGARLAYDHLALTTGARVRKLDCKGA
SHDAVHYLRDLRDARRLVERTQTARRAVVVGGGYIGLEAAASLRQKGLDVTVVETEARVL
ARVASPSISVIMQRAHTRHGVSLALGRKVVAIHDVADGVAVELDDGARLSCDLVVVGIGV
LPNTELAAGCGLDVAGGIVVDACARTSDPSIVAAGDCAAFVPYWAPTGSAACRIESVQNA
NDMARTAAASVLGRSEPYRAVPWFWSDQYDLKLQMAGVNTGFTDFAVRGSVDEGKFSLFY
FRDDRLVAVDSINRPQDHMLARKLLSTGTRLSVEDVNGPGFDLKAFANRDLGVAHGAAS