Protein Info for H281DRAFT_03661 in Paraburkholderia bryophila 376MFSha3.1

Annotation: phospholipase C

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 720 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 5 to 25 (21 residues), 17 bits, see alignment (E = 5.7e-07) TIGR03396: phospholipase C, phosphocholine-specific" amino acids 5 to 711 (707 residues), 919.7 bits, see alignment E=1.1e-280 PF04185: Phosphoesterase" amino acids 48 to 435 (388 residues), 392.6 bits, see alignment E=1.8e-121 PF05506: PLipase_C_C" amino acids 506 to 592 (87 residues), 89.9 bits, see alignment E=1.8e-29 amino acids 618 to 699 (82 residues), 77.1 bits, see alignment E=1.7e-25

Best Hits

KEGG orthology group: K01114, phospholipase C [EC: 3.1.4.3] (inferred from 94% identity to bug:BC1001_4712)

Predicted SEED Role

"Phospholipase C 4 precursor (EC 3.1.4.3)" (EC 3.1.4.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.3

Use Curated BLAST to search for 3.1.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MLJ7 at UniProt or InterPro

Protein Sequence (720 amino acids)

>H281DRAFT_03661 phospholipase C (Paraburkholderia bryophila 376MFSha3.1)
MTSNSRRRFLQTVASSAGAAAALTALPESIRNALAVPAFSRTGTIRDVEHIVVFMQENRS
FDHYFGHLRGVRGYNDRFPIPLPSGKPVWYQPSKADPTKPVLPFHLDTSTTSAQCVGDLD
HSWYKTHAAIDGGRYDQWPATKTDMTMGYHLRGDIPFHYALADAFTVCDAYFCSLPGPTH
PNRSYLMTGTVDPTGKFGGPLLDNNDWVDGDGPPNYQLLSWTTYPERLQAAGISWQVYQQ
GLTGSDPLNGNYGTNILQNFSNFINAQPGSPLYERAQTVRTIDDLKADVLANKLPQVSWL
CPPAAYSEHPQYTPAYGAEYTSQILDALTSNPEVWSKTVLFIMYDENDGFFDHMVPPQPA
TTPAQGKSTVSTEGEIHNVVNPGRGGSYTADGLPYGLGPRVPMTIVSPWSKGGFVCSQVF
DHTSVIRFIETRFGVYEPNITAWRRAVCGDLTTAFDFRTPDSKVPPLPDTSNYKSIADNQ
CATQPKPTVPATPGAIDPQEGGIRFARALPYELHVNGHADPKKSTFELSIGNTGDQGAHF
YVYSTNRTDGPWRYTVEAGKSLSESFDLTTTNGAYAFEVFGPSGFVRKFAGNVAATAQGA
TQASQFGAGHHNEKPAQPEVKVQYDVANGNVFLKFSNKGDKLARLTVTDNAYGARPRPVL
VPAGAHIEEAWVLAASHHWYDLTVTSNDDESFLRRVAGHVENGRPSISDPAAIAPVLVAN