Protein Info for H281DRAFT_03609 in Paraburkholderia bryophila 376MFSha3.1

Annotation: squalene-associated FAD-dependent desaturase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 PF13450: NAD_binding_8" amino acids 1 to 57 (57 residues), 45.9 bits, see alignment 8.7e-16 TIGR03467: squalene-associated FAD-dependent desaturase" amino acids 1 to 409 (409 residues), 358.3 bits, see alignment E=3.9e-111 PF00890: FAD_binding_2" amino acids 1 to 33 (33 residues), 26.8 bits, see alignment (E = 4.6e-10) PF01593: Amino_oxidase" amino acids 6 to 409 (404 residues), 133.1 bits, see alignment E=2.7e-42

Best Hits

Swiss-Prot: 52% identical to HPNE_RHOPA: Hydroxysqualene dehydroxylase (hpnE) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: None (inferred from 82% identity to bug:BC1001_4760)

MetaCyc: 52% identical to hydroxysqualene dehydroxylase (Rhodopseudomonas palustris CGA009)
RXN-17128 [EC: 1.17.8.1]

Predicted SEED Role

"Phytoene desaturase (EC 1.14.99.-)" in subsystem Carotenoids (EC 1.14.99.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.99.-

Use Curated BLAST to search for 1.14.99.- or 1.17.8.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5ML37 at UniProt or InterPro

Protein Sequence (416 amino acids)

>H281DRAFT_03609 squalene-associated FAD-dependent desaturase (Paraburkholderia bryophila 376MFSha3.1)
VVGAGLAGLAAAVQLQRRGARVVLHEAAQQAGGRCRSYYDAKLGATIDSGNHLMMSGNAA
TLNYARAIGAVDELTGPALPEFPFVDLATHARWTVRLSSGRLPWWIFDQNARVPNTGPSD
YLSLVPLLFAKPGRTVAQTMRSNGPLWDRLLAPLFRAMLNAEPRDASAALTGAMVRETLM
AGGLACRPLIARNGLSSAFVDPALRLLQHGGATIEIGSRLEKMRFAANGSRVEALDFAEQ
SVAMDVNQAVILAVPPDVAQTLVPGLRAPARFAATLSAYFAVEPPFGLPPVTGLLNGTAQ
WLFASDGRLSATVYGVGNVVDTPREELAARLWAEVAQAANLPAAPMPAWQIVVDERATFA
ASPEQETLRPGTRTRWNNLMLAGDWTATGLPATIEGAIRSGQKAADTLLNQPMERR