Protein Info for H281DRAFT_03236 in Paraburkholderia bryophila 376MFSha3.1

Annotation: thioredoxin reductase (NADPH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 557 PF00027: cNMP_binding" amino acids 40 to 124 (85 residues), 54.7 bits, see alignment E=5.5e-18 PF01946: Thi4" amino acids 238 to 276 (39 residues), 28.4 bits, see alignment 6.2e-10 PF07992: Pyr_redox_2" amino acids 238 to 542 (305 residues), 122.2 bits, see alignment E=1.9e-38 PF03486: HI0933_like" amino acids 238 to 270 (33 residues), 25.1 bits, see alignment (E = 4.8e-09) PF01134: GIDA" amino acids 239 to 267 (29 residues), 20.2 bits, see alignment (E = 1.8e-07) PF01266: DAO" amino acids 239 to 280 (42 residues), 30 bits, see alignment 2.7e-10 PF00890: FAD_binding_2" amino acids 239 to 276 (38 residues), 28.5 bits, see alignment 6.2e-10 PF12831: FAD_oxidored" amino acids 239 to 275 (37 residues), 27.2 bits, see alignment 1.7e-09 PF13450: NAD_binding_8" amino acids 242 to 280 (39 residues), 28.4 bits, see alignment 1.1e-09 PF13738: Pyr_redox_3" amino acids 326 to 496 (171 residues), 44.8 bits, see alignment E=6.8e-15 PF00070: Pyr_redox" amino acids 384 to 455 (72 residues), 33.1 bits, see alignment E=4.4e-11

Best Hits

KEGG orthology group: K00384, thioredoxin reductase (NADPH) [EC: 1.8.1.9] (inferred from 95% identity to bug:BC1001_4089)

Predicted SEED Role

"Thioredoxin reductase (EC 1.8.1.9)" in subsystem Glycine reductase, sarcosine reductase and betaine reductase or Thioredoxin-disulfide reductase or Wyeosine-MimG Biosynthesis (EC 1.8.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.9

Use Curated BLAST to search for 1.8.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MIL9 at UniProt or InterPro

Protein Sequence (557 amino acids)

>H281DRAFT_03236 thioredoxin reductase (NADPH) (Paraburkholderia bryophila 376MFSha3.1)
MSDKTSGKREDLLAGGSRHHQIFPRLNDAQFAVLERYGERRKLSAGEVLFTEGDRHVPMF
AIVSGTIEATRGSGENLHFLGTHGPGSFTGEVGTLAGRGAVASTRATSDCEVIVIDEESL
RALVVAEAELSETIMRAYILRRVAYIQDQSGGVVVIGSSGSFPTLVLRHFLSRNAQPSAY
FDIAEHEEAKALLDRYGATEEDLPVLVTLQGTILKRPTNRAVADAIGLSPDRLNGERFDV
VVVGAGPAGLAAAVYAASEGLRVAVLDTKAPGGQAGTSSKIENYFGFPTGISGQALAGRG
LSQCRKFGAEVGVPIEALGIECDKGQPFHIRLNYDEHVYARAVVIATGARYRKPELERLE
HFQGRGVYYSATFMEAAFCGNEELVIVGGGNSAGQAAVFLSGFARHVHIVIRGEGLNASM
SSYLIRRIEAAPNITVHVRTQIVALNGEEHLESIQWNQQGRIEEKAIRHVFLFLGAQPST
GWLGDCVELDKNGFVLTGPDVAHKWTSERPAHFLETSRPGIFAVGDVRSGSVKRVAAAVG
EGAAAIQSLHQYLALAP