Protein Info for H281DRAFT_02908 in Paraburkholderia bryophila 376MFSha3.1

Annotation: RNA polymerase, sigma 54 subunit, RpoN/SigL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 PF00309: Sigma54_AID" amino acids 4 to 47 (44 residues), 55.8 bits, see alignment 4.8e-19 TIGR02395: RNA polymerase sigma-54 factor" amino acids 9 to 491 (483 residues), 454.9 bits, see alignment E=1.7e-140 PF04963: Sigma54_CBD" amino acids 128 to 321 (194 residues), 194.2 bits, see alignment E=2.7e-61 PF04552: Sigma54_DBD" amino acids 336 to 492 (157 residues), 204.3 bits, see alignment E=1.5e-64

Best Hits

Swiss-Prot: 47% identical to RP54_CUPNH: RNA polymerase sigma-54 factor (rpoN) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K03092, RNA polymerase sigma-54 factor (inferred from 97% identity to bug:BC1001_3735)

Predicted SEED Role

"RNA polymerase sigma-54 factor RpoN" in subsystem Flagellar motility or Flagellum or Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MJQ4 at UniProt or InterPro

Protein Sequence (493 amino acids)

>H281DRAFT_02908 RNA polymerase, sigma 54 subunit, RpoN/SigL (Paraburkholderia bryophila 376MFSha3.1)
MPSIELRTRQSLALTPRLQQSVRLLQLSSLEFQQELRTALDTNPFLEYDSSDTEDVALAT
ATSGEEQGVLPATDAVAAAEPDSLPSEAASSESLDSGGPDDMPGDFSGDYSSRSSARQNG
DSDGSDPAEWARSQPTLREQLHDALRLYRLDDRDRAVARFIIEALDDDGYLRQDLADLAD
SVDLEPELTEEELLVALRLVQSLDRPGLGARSLSECLLLQVNALPADTPGRDVARQIVEH
HLERLARREQAELQKQIGCTAEELRVACALVRKLDPKPGNCYGRTEDNYVVPDVIVRQVR
NKWVVTINPAVQPRARIHRMYAELFAQSAGASRSPLAQQLQEARWLIRNAQQRFDTIQRV
AECIVAHQKAFFQYGEIALKPMVLRDVADELGLHESTISRATGNKYMATPRGIFEFKHFF
PRELGTESGGTCSAAAVRALLKEMIAAENTRDPLSDVTLAKMLADQGVLVARRTVAKYRH
LMKVPPAELRRQV