Protein Info for H281DRAFT_02819 in Paraburkholderia bryophila 376MFSha3.1

Annotation: DNA ligase-1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details TIGR04120: DNA ligase, ATP-dependent, PP_1105 family" amino acids 2 to 555 (554 residues), 726.7 bits, see alignment E=1e-222 PF04675: DNA_ligase_A_N" amino acids 2 to 161 (160 residues), 44.8 bits, see alignment E=2.6e-15 PF01068: DNA_ligase_A_M" amino acids 225 to 427 (203 residues), 104.9 bits, see alignment E=7e-34 PF04679: DNA_ligase_A_C" amino acids 448 to 541 (94 residues), 39.3 bits, see alignment E=1e-13

Best Hits

KEGG orthology group: K01971, DNA ligase (ATP) [EC: 6.5.1.1] (inferred from 93% identity to bgf:BC1003_3707)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MIJ0 at UniProt or InterPro

Protein Sequence (561 amino acids)

>H281DRAFT_02819 DNA ligase-1 (Paraburkholderia bryophila 376MFSha3.1)
MKRFAALYTALDATTSTHDKLEALTSYFAVAEPEDAAWASYFLAGGKPRQSVPTRLLTEF
ARERAGLPAWLFEESYHAVGDLAETIAHILPPAQRSSELGLTQWIEQRVLTLRGLAPEEL
RTRLFSYWDELDWGERFLLTKLIGGGFRVGVARQLVVRALAQVAGVDHKRIAQRMVGWTD
SRQKPDAARYLRLIAPDAPDAGNAGEQQSADRHDSDLGLPYPFFLAHPLQTDPETLGDPS
RWLIEWKWDGIRAQLVKRGGRVWLWSRGEDLITDRFPEVAALGEALPDGFVIDGEILAWE
PGAAAPLPFARLQPRIARKTLTKKILADSPAALLAYDLLEAHGEDLRMTPLAERRARLDA
LAASLDGTLSGDLLRVSPQVSGADWRALAALREESRARGVEGFMVKERESMYGVGRTKAS
GTWWKWKIDPYAIDAVLVYAQRGHGRRASLYTDFTFAVWDEANGVRTLVPFAKAYSGLTD
EEMRQVDAIVRKTTIEKFGPVRSVTPTLVFEIGFEGIQASTRHKSGVAVRFPRMLRWRTD
KHIDDADTLAMLKGFIDDRAA