Protein Info for H281DRAFT_02594 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 3-carboxy-cis,cis-muconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00206: Lyase_1
  • amino acids 25 to 306 (282 residues), 186.1 bits, E=1.1e-58
• PF10397: ADSL_C
  • amino acids 372 to 449 (78 residues), 73 bits, E=2.1e-24

Best Hits

Swiss-Prot: 47% identical to YBN5_SCHPO: Uncharacterized protein C8E4.05c (SPBC8E4.05c) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 88% identity to rsl:RPSI07_mp0965)

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (22/35 steps found)
• superpathway of aerobic toluene degradation (16/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 5.5.1.2

Use Curated BLAST to search for 5.5.1.2

Protein Sequence (456 amino acids)

>H281DRAFT_02594 3-carboxy-cis,cis-muconate cycloisomerase (Paraburkholderia bryophila 376MFSha3.1)
MTTRTTPLSSTVFDSILFRDAFGTAKMRALFSDYALVQRYIDVEVALAKAEARVGVIPTD
AADVIARESQIERIDFDHMREETDIVGYPILPLVHQLVGMCGEAGRYVHWGATTQDIMDT
AVALQVRDALDSIDADIRELRGILVDLSKKHRDTPMAGRTHLQQALPVTFGYKTAIWLAM
FDRHQQRIAQLRPRVAVVEFAGAAGTLASLGDKGFDVQKALAAELQLGVPATTWHVARDG
FAEAVNLLALVTGSLGKIATDIMIMASNEFGEVYEPFVKGRGASSTMPQKRNPISSELML
AAAKAVRQQAGLMVDAMIQDFERATGPWHAEWIAIPESFILASGALHQAKFALGGLIVDT
DRMKHNLGISKGLIVAEAVMMQMAPYTGRQQAHDIVYDACRTVNESGGTLAEALAALPEV
TRHFDRDAINAMTDPANYLGLAPQMVDRAIALSSEI