Protein Info for H281DRAFT_02540 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00171: 3-isopropylmalate dehydratase, small subunit
  • amino acids 1 to 178 (178 residues), 158.7 bits, E=7.3e-51
• PF00694: Aconitase_C
  • amino acids 49 to 116 (68 residues), 77 bits, E=9.2e-26

Best Hits

Swiss-Prot: 46% identical to LEUD_ERYLH: 3-isopropylmalate dehydratase small subunit (leuD) from Erythrobacter litoralis (strain HTCC2594)

KEGG orthology group: K01704, 3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 52% identity to adn:Alide_3500)

MetaCyc: 41% identical to isopropylmalate isomerase small subunit (Leptospira interrogans serovar Lai str. 56601)
(R)-2-methylmalate dehydratase. [EC: 4.2.1.35]; 3-isopropylmalate dehydratase. [EC: 4.2.1.35, 4.2.1.33]; 4.2.1.33 [EC: 4.2.1.35, 4.2.1.33]; RXN-7744 [EC: 4.2.1.35, 4.2.1.33]

Predicted SEED Role

"3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (200 amino acids)

>H281DRAFT_02540 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33) (Paraburkholderia bryophila 376MFSha3.1)
MERFQTVSGPAAPLLRRDVDTDIIIRIERLTGLPKDQLGQYAFEVWRGPDFVLDREPFVG
APILIAGANFGCGSSREAAVWALQGLGLRVVIAPSFGEIFMSNCFQNGVLPVVLPEQQVA
NLAEQAQSGAAVTADLDTCVITAPDGTSFPFQVDPVRREMLIEGADEMSLTLRRQDLIRQ
WQADDARRRPWVYVGSRSAD