Protein Info for H281DRAFT_02398 in Paraburkholderia bryophila 376MFSha3.1

Annotation: fatty-acyl-CoA synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 38 to 429 (392 residues), 321.5 bits, E=6.9e-100
• PF13193: AMP-binding_C
  • amino acids 479 to 554 (76 residues), 77.2 bits, E=1.5e-25

Best Hits

Swiss-Prot: 47% identical to ACSF2_BOVIN: Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) from Bos taurus

KEGG orthology group: None (inferred from 93% identity to bgf:BC1003_3446)

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism or Biotin biosynthesis or n-Phenylalkanoic acid degradation (EC 6.2.1.16, EC 6.2.1.3)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• oleate β-oxidation (29/35 steps found)
• fatty acid salvage (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• octane oxidation (4/5 steps found)
• long-chain fatty acid activation (1/1 steps found)
• 2-deoxy-D-ribose degradation II (6/8 steps found)
• phytol degradation (3/4 steps found)
• stearate biosynthesis IV (4/6 steps found)
• γ-linolenate biosynthesis II (animals) (1/2 steps found)
• linoleate biosynthesis II (animals) (1/2 steps found)
• capsaicin biosynthesis (4/7 steps found)
• alkane biosynthesis II (1/3 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• long chain fatty acid ester synthesis (engineered) (1/4 steps found)
• phosphatidylcholine acyl editing (1/4 steps found)
• wax esters biosynthesis II (1/4 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• stearate biosynthesis I (animals) (1/6 steps found)
• arachidonate biosynthesis III (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis II (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis III (8-desaturase, mammals) (1/7 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• sporopollenin precursors biosynthesis (4/18 steps found)
• cutin biosynthesis (1/16 steps found)
• suberin monomers biosynthesis (3/20 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.3

Use Curated BLAST to search for 6.2.1.16 or 6.2.1.3

Protein Sequence (576 amino acids)

>H281DRAFT_02398 fatty-acyl-CoA synthase (Paraburkholderia bryophila 376MFSha3.1)
MATQVSGEGAIIEPKDGLSYVRGSTEIPLSEATIGEFLRRTAERFPDRPAVVFREQQIRW
TWKEFAEEVDILAAGFLALGIVKGDRVGIWSPNRVEWLLTQFATARIGAVLVNINPAYRL
AELEYALNKVGCKAIVAAERFKTSMYLEMLQQLAPELATHAPGDLHAARLPDLRYVIRMC
DTETPGMLTFSDVIEQGRATLDVAKLDEIGATLSCHEAINIQFTSGTTGNPKGATLTHSN
VVNNARYIAMAMNLSEQDALCIPVPLYHCFGMVLAVLACVSVGANMVFPGEAFDPAATLA
AVAQEQCTALHGVPTMFIAELDHPDFASFDLSRLRTGIMAGSPCPIETMKKVVSRMHLNE
ITIAYGMTETSPVSFQSSTTDPLDKRTTTVGRIQPHLEVKIVDPVGDIVPVSETGELCTR
GYSVMQGYWDDEPKTRESIVDGWMHTGDLATLDAEGYCNIVGRLKDMLIRGGENIYPREI
EEFLFRHPKIQSVQVFGVPDSRYGEEVCAWVVLRPGEQATAEEIQQFCNGQIAHYKVPKY
IRFVDELPMTVTGKVQKFVMRERMISELKLQEGKTA