Protein Info for H281DRAFT_02373 in Paraburkholderia bryophila 376MFSha3.1
Annotation: methylmalonate-semialdehyde dehydrogenase [acylating]
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to BAUC_PSEAE: Putative 3-oxopropanoate dehydrogenase (bauC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 96% identity to bug:BC1001_3543)MetaCyc: 68% identical to malonate-semialdehyde dehydrogenase (acetylating) (Halomonas sp. HTNK1)
Malonate-semialdehyde dehydrogenase (acetylating). [EC: 1.2.1.18]
Predicted SEED Role
"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)
MetaCyc Pathways
- myo-inositol degradation I (6/7 steps found)
- β-alanine degradation II (2/2 steps found)
- β-alanine degradation I (1/2 steps found)
- L-valine degradation I (5/8 steps found)
- myo-, chiro- and scyllo-inositol degradation (6/10 steps found)
- acrylate degradation I (2/5 steps found)
- propanoyl-CoA degradation II (2/5 steps found)
- 2,4-dinitrotoluene degradation (1/7 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Inositol phosphate metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.27
Use Curated BLAST to search for 1.2.1.18 or 1.2.1.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2Z5MEY9 at UniProt or InterPro
Protein Sequence (512 amino acids)
>H281DRAFT_02373 methylmalonate-semialdehyde dehydrogenase [acylating] (Paraburkholderia bryophila 376MFSha3.1) MSETYQEEGVRADAGTRALTHFINGKAIDGTSGRFGDVFNPALGDVAARVPLASVAEVDA AVAAAYAAFPAWSETAPIKRARVLFKFKELLDRHHDELAELITREHGKVFSDAKGEVMRG IEVVEFACGIPNLLKTDFTDQIGGGIDNWNLRQPLGVVVGITPFNFPMMVPCWMFPVAIA CGNTFVLKPSERDPSASNRLAELLKEAGLPDGVFNVVHGDKVAVDALLVHPDVSAVSFVG STPIAEYIHTEGTKHGKRVQALGGAKNHLVVMPDADLDQAVDALIGAAYGSAGERCMAIS VAVAVGHIADELIERLTPRVESLKIMNGMESAAEMGPLVTAVHREKVVGYIEAGIAEGAK LVVDGRGHRVDGHEKGFFLGGTLFDDVSTDMKIYREEIFGPVLCVVRVPDFASAVELINA NDFANGVSLFTSDGGIARAFSRQIQIGMVGINVPIPVPMAWHSFGGWKKSLFGDHHAYGE EGVRFYTRYKSIMQRWPDSIAKGAEFTMPVAK