Protein Info for H281DRAFT_02244 in Paraburkholderia bryophila 376MFSha3.1

Annotation: DNA topoisomerase-3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 TIGR01056: DNA topoisomerase III" amino acids 3 to 616 (614 residues), 561.2 bits, see alignment E=2e-172 PF01751: Toprim" amino acids 5 to 131 (127 residues), 35.6 bits, see alignment E=1.3e-12 PF01131: Topoisom_bac" amino acids 153 to 595 (443 residues), 382.5 bits, see alignment E=4.2e-118 PF13342: Toprim_Crpt" amino acids 645 to 708 (64 residues), 62.7 bits, see alignment 3.8e-21 amino acids 756 to 816 (61 residues), 66.8 bits, see alignment 2e-22

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 89% identity to bgl:bglu_1g34130)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M7Q4 at UniProt or InterPro

Protein Sequence (889 amino acids)

>H281DRAFT_02244 DNA topoisomerase-3 (Paraburkholderia bryophila 376MFSha3.1)
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDEYVLSSAVGHLLEIAAPDEYEVKRGK
WSFANLPVIPPHFDLNPIAKSESRLKVLTKLLKRKDIDRLINACDAGREGELIFRLIAQH
AKAKQPVQRLWLQSMTPAAIRDGFARLRSDEEMQPLADAARCRSEADWLVGINGTRAMTA
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAEFVCAAGFYEGRWFDP
KFKRDEFDPEKRDSRLWALPAAETIVAACRGQIGTVSEESKPSTQLSPALFDLTSLQREA
NGRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYMDTVKQTLGMLKESNNYLPFA
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKNLSEPEQKLYDLVVKRFLSVFFPAA
EFKVTTRITEVVGHHFKTEGKVLVEPGWLQVYGREISGEDANLVPVQKDEKVKTDKIAAQ
QLVTKPPARYNEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLI
REGRDLIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLPRDAFMQEIARM
TQTIVKRAKEYDSDTIPGDYATLQTPCPHCGGQVKENYRRFACSKCEFSISKIPGGRQFE
IEEVEELLKNKTIGPLSGFRSKMGRPFSAILKLSLDDEIENYKLEFDFGQDAGGEDGEPP
DFSDQESVGACPKCKGRVFEHGMSYVCENSVASPKTCDFRSGKVILQQEIAREQMAKLLE
EGRTDLLTNFKSSRTGRNFKAFLVKQNDGKIGFEFEKKEPSAKTAAKTAAAKSAAKAGAE
LESDEGDATVAVKAAPAAKKAAAKKAPATKTAAAKKAPAKKTAARKTGS