Protein Info for H281DRAFT_02231 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 7-carboxy-7-deazaguanine synthase, Cx14CxxC type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 210 TIGR04508: 7-carboxy-7-deazaguanine synthase" amino acids 3 to 210 (208 residues), 367.7 bits, see alignment E=9.5e-115 PF04055: Radical_SAM" amino acids 46 to 128 (83 residues), 33.1 bits, see alignment E=3.1e-12

Best Hits

Swiss-Prot: 91% identical to QUEE_BURM1: 7-carboxy-7-deazaguanine synthase (queE) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: None (inferred from 96% identity to bge:BC1002_0034)

Predicted SEED Role

"Queuosine Biosynthesis QueE Radical SAM" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (210 amino acids)

>H281DRAFT_02231 7-carboxy-7-deazaguanine synthase, Cx14CxxC type (Paraburkholderia bryophila 376MFSha3.1)
MTYAVKEIFYTLQGEGANAGRPAVFCRFAGCNLWSGREEDRAEAVCRFCDTDFVGTDGEN
GGKYRTAEDLVQMIASQWPEGEGQRFVVCTGGEPMLQIDQPLVDALHAAGFEIAIETNGS
LPVLETIDWICVSPKADAPLVVTKGNELKVVIPQDNQRLSDYAKLDFEYFLVQPMDGPSR
DINTKLAIDWCKRHPQWRLSMQTHKYLNIP