Protein Info for H281DRAFT_02108 in Paraburkholderia bryophila 376MFSha3.1

Annotation: haloacetate dehalogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 PF00561: Abhydrolase_1" amino acids 27 to 244 (218 residues), 82.3 bits, see alignment E=6.8e-27 PF12697: Abhydrolase_6" amino acids 29 to 285 (257 residues), 46.1 bits, see alignment E=1.5e-15 PF03096: Ndr" amino acids 40 to 134 (95 residues), 23.6 bits, see alignment E=3e-09

Best Hits

Swiss-Prot: 47% identical to DEHA_RHOPA: Fluoroacetate dehalogenase (RPA1163) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K01561, haloacetate dehalogenase [EC: 3.8.1.3] (inferred from 93% identity to bge:BC1002_0177)

MetaCyc: 45% identical to fluoroacetate dehalogenase monomer (Moraxella sp. B)
Haloacetate dehalogenase. [EC: 3.8.1.3]

Predicted SEED Role

"Haloacetate dehalogenase H-1 (EC 3.8.1.3)" (EC 3.8.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.8.1.3

Use Curated BLAST to search for 3.8.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (296 amino acids)

>H281DRAFT_02108 haloacetate dehalogenase (Paraburkholderia bryophila 376MFSha3.1)
MAFETFTPFRVAAGDADIFGVKGGAGPPLLLLHGHPQSHLIWARCADQLAQHFTVIATDL
RGYGASAKPQSDATHAPYSKRSMAADQVAVMRHFGFERFLVCAHDRGARVAHRMALDHAD
AVERLMLLDIAPTLAMYEATDRTFATHYFHWFFLIQPEPLPETLIGANPAAYVDAVMGSR
HAGLAPFDPASLDAYRSALAQPGAVHAMCEDYRASASIDLEHDRADIERGQKIACPLRVL
WGDKGVIEKCFDALDEWRHVARDVSGRSLSCGHYIPEEAPDELVAEMLSFFEALEK