Protein Info for H281DRAFT_02101 in Paraburkholderia bryophila 376MFSha3.1

Annotation: hydroxymethylglutaryl-CoA lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00682: HMGL-like
  • amino acids 6 to 280 (275 residues), 185.4 bits, E=7.7e-59

Best Hits

Swiss-Prot: 61% identical to LIUE_PSEAE: 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methylglutaryl-CoA lyase (liuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 97% identity to bxe:Bxe_A4270)

MetaCyc: 61% identical to hydroxymethylglutaryl-CoA lyase subunit (Pseudomonas aeruginosa PAO1)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]; 3-hydroxy-3-isohexenylglutaryl-CoA lyase. [EC: 4.1.3.4, 4.1.3.26]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• mevalonate degradation (2/2 steps found)
• ketogenesis (3/5 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Geraniol degradation
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.4

Use Curated BLAST to search for 4.1.3.26 or 4.1.3.4

Sequence Analysis Tools

See A0A2Z5M8G3 at UniProt or InterPro

Protein Sequence (308 amino acids)

>H281DRAFT_02101 hydroxymethylglutaryl-CoA lyase (Paraburkholderia bryophila 376MFSha3.1)
MAVPKQVKIVEVGPRDGLQNEKEFVPTATKIELINRLSAAGFRNVEAASFVSPKWVPQMA
DGAEVMSGIERRPGTIYSVLTPNLRGFEGALAARADEIVIFGAASEAFSQKNINCSIAES
IERFAPVAQAAKEHGLRIRGSVSCALGCPYQGEVPVASVVDVVQRFAALGCDEIDIADTI
GVGTPKRTREVFEAVTQVFPRERLSGHFHDTYGQALANIYAALQEGIEIYHASVAGLGGC
PYAKGATGNVATEDVLYLMNGLGIETGIDLAQVVAIGDFISTSIGRPNVSRAGKALLAKA
RSEAANCV