Protein Info for H281DRAFT_02091 in Paraburkholderia bryophila 376MFSha3.1
Annotation: hypothetical protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 46% identical to ACADS_PIG: Short-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADS) from Sus scrofa
KEGG orthology group: None (inferred from 97% identity to bpy:Bphyt_0459)MetaCyc: 65% identical to short-chain acyl-CoA dehydrogenase monomer (Pseudomonas putida KT2440)
RXN-13449 [EC: 1.3.8.1]
Predicted SEED Role
"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)
MetaCyc Pathways
- oleate β-oxidation (29/35 steps found)
- superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- β-alanine biosynthesis II (4/6 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- valproate β-oxidation (6/9 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.3.8.1, 1.3.99.2
Use Curated BLAST to search for 1.3.8.1 or 1.3.99.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2Z5M804 at UniProt or InterPro
Protein Sequence (378 amino acids)
>H281DRAFT_02091 hypothetical protein (Paraburkholderia bryophila 376MFSha3.1) MVLDQDHLMVRDALRTFVREAVTPYAATWDRERTFPKDVHRQLAELGAYGVLVPETYGGA GMDALALALILEEIAAGDGGTSTAISVNNCPVCSILLTYGNDAQKREWLTPLARGEMLGA FCLTEPQAGSDASALRTTATRDKDGDAYVLNGVKQFITSGKNGDVAIVMAVTDKAAGKRG ISAFIVPTDSKGYVVARVEDKLGQHSSDTAQIIFEDCRVPAANLIGAEGEGYRIALSGLE GGRIGIAAQSVGMARAAYEAALTYAKERESFGQPLFSHQAVQFRLADMATQLEAARQLIW HAASLKDAGQPCLTEAAMAKLFASEAAERICSAALQIHGGYGYLSDFPVERIYRDVRVCQ IYEGTSDIQKILIARGLG