Protein Info for H281DRAFT_02087 in Paraburkholderia bryophila 376MFSha3.1

Annotation: isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 transmembrane" amino acids 325 to 347 (23 residues), see Phobius details PF20423: AceK_regulatory" amino acids 17 to 321 (305 residues), 398.1 bits, see alignment E=2.4e-123 PF06315: AceK_kinase" amino acids 322 to 576 (255 residues), 401.6 bits, see alignment E=1.6e-124

Best Hits

Swiss-Prot: 92% identical to ACEK_PARPJ: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 93% identity to bgf:BC1003_0204)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M9F6 at UniProt or InterPro

Protein Sequence (615 amino acids)

>H281DRAFT_02087 isocitrate dehydrogenase kinase/phosphatase (Paraburkholderia bryophila 376MFSha3.1)
MNHFPKLLSSQIGFDVAETLLEGFNRHYRIFRDAAIHAKALFESGDWHGLQKLARERITS
YDDRVEECVALLEDEYDAENIDSEVWQQIKLHYIGLLTTHRQPECAETFFNSVCCKILHR
SYFNNDFIFVRPAISTEYIENDEPAAKPTYRAYYPGKDGLAATLERIVTNFQLEPPFEDL
TRDVGCVMQTIHDSFGTFDEAPNFQIHVLSSLFFRNKSAYIVGRIINGDMLLPFAVPLRH
VQPGLLALDTVLLKREQLLIIFSFSHSYFLVDMEVPSAYVEFLGTIMPGKPKAEIYTSVG
LQKQGKNLFYRDLLHHLSHSSDQFIIAPGIKGLVMLVFTLPSFPYVFKLIKDNFPPPKET
TRAKIQEKYQLVKRHDRLGRMADTLEYSSVALPLSRLDEALLRELEKEVPSLLEYDGDNL
VIRHMYIERRMVPLNLFLQNGSDNDVDHGIKEYGNAVKELMQANIFPGDMLYKNFGVTRH
GRVVFYDYDEIEYLTDCNVRAVPAPRNEEDEMSGEPWYSVGPHDIFPETYGTFLLGDPRV
RRSFLQHHADFFDPALWQKHKDHLLKGELPDFFPYDSNVRFCIRYPERFADTASNAPNAQ
DAAQAPENERSVKVA