Protein Info for H281DRAFT_01854 in Paraburkholderia bryophila 376MFSha3.1

Annotation: isoamylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 696 transmembrane" amino acids 512 to 528 (17 residues), see Phobius details TIGR02100: glycogen debranching enzyme GlgX" amino acids 17 to 693 (677 residues), 643.4 bits, see alignment E=1.9e-197 PF02922: CBM_48" amino acids 23 to 114 (92 residues), 34.4 bits, see alignment E=3.4e-12 PF00128: Alpha-amylase" amino acids 182 to 531 (350 residues), 59.8 bits, see alignment E=5.4e-20 PF21156: ISOA1-3_C" amino acids 600 to 673 (74 residues), 44 bits, see alignment E=3.5e-15

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 75% identity to bge:BC1002_1248)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (696 amino acids)

>H281DRAFT_01854 isoamylase (Paraburkholderia bryophila 376MFSha3.1)
LSNAERGPAPGYPVGPGSRFPPGATVVPEGVNFCIFGRHATQVELLLYAAPDSPEPFQVI
TLSSERNRTFFYWHVLVLDLPIRTCYTWRVDGAQDTEHTGRAFYPHRELLDPHARAVSDD
LWKRTSASAILETGRASYRAIVTQPVPADATPVASGLDGAVIYELHVGGFTSHPSSNVTH
PGTFAGLIEKIPYLTQLGVTHVELLPVMAFDAQDVPPAAAARGLCNYWGYSTHSFYSPHP
RYCVDPASAPQEFRALTDAMHAAGIGVLLDVVFNHTAEAGASGPVINFKGLANDIFYHLD
AGDRRRYRDYTGCGNTVNCNHPLVTAFIVRCLEYWVQELGVDGFRFDLASVFARDQHGDL
LSDPPLPWAIESSRILSRVPLIAEAWDAGGLYQVGAFPGMAWAEWNGRYRDVIRRFVRGD
PGMCGEVATCIAGCADLYADDGRLPANSINFITCHDGFTLNDLVSYNAKHNEVNGEENRD
GSNDNLSWNCGAEGETDDAGVRRLRSQQARNLMAILFLSQGVPMILAGDELLRSQRGNNN
GYSQNNVLSWIDWRSIEAESGMLRFVRELIALRKRHASLRRRRFLTGRPSSGHANPDVAW
HGERLNEPRWHDPGVRLLAFTLGGEASGESALHVMLNMDDCARRFDLPVLDGRRWCRIID
TAQESPNDIVSTLQHAAIESETCCVDARSVVVLEAI