Protein Info for H281DRAFT_01852 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Phosphocarrier protein HPr/phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9)/PTS system IIA component, Glc family (TC 4.A.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 851 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00358: PTS_EIIA_1" amino acids 11 to 135 (125 residues), 143.4 bits, see alignment E=7.7e-46 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 12 to 132 (121 residues), 122 bits, see alignment E=2.1e-39 TIGR01003: phosphocarrier, HPr family" amino acids 176 to 254 (79 residues), 69.9 bits, see alignment E=2.1e-23 PF00381: PTS-HPr" amino acids 176 to 255 (80 residues), 85.7 bits, see alignment E=4.5e-28 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 297 to 846 (550 residues), 549.5 bits, see alignment E=1.2e-168 PF05524: PEP-utilisers_N" amino acids 298 to 414 (117 residues), 90.7 bits, see alignment E=2.1e-29 PF00391: PEP-utilizers" amino acids 439 to 512 (74 residues), 83.3 bits, see alignment E=2e-27 PF02896: PEP-utilizers_C" amino acids 539 to 821 (283 residues), 364.1 bits, see alignment E=1.3e-112

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 79% identity to bge:BC1002_5515)

Predicted SEED Role

"PTS system, glucose-specific IIA component (EC 2.7.1.69) / Phosphocarrier protein of PTS system / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Trehalose Uptake and Utilization or Fructose and Mannose Inducible PTS or Mannitol Utilization or Fructose utilization (EC 2.7.1.69, EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (851 amino acids)

>H281DRAFT_01852 Phosphocarrier protein HPr/phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9)/PTS system IIA component, Glc family (TC 4.A.1) (Paraburkholderia bryophila 376MFSha3.1)
MKALQRPLRIELVAPLSGVMVPLETVPDPVFAQKMVGDGISIDPTSHELLSPLPGKVTQL
HSSSHAVTITGASGLEVLLHIGLDTVLLRGEGFTPLVKEGDTVATGQPLIRFDPVYVGAK
AASLLTQMVIANGDRVTRYVPAEGLVTAAGDVALTVELADDTAKDQTARTTGAIVSDEVT
LPNPAGMHARPAAVFVGAAKKYESEIRLLHGSNSANAKSIVSIMGLATKFGDRVRIQATG
PDAGEAASVLARLLAEGSGEKPADAPAFPPTSLAPASGSSGEPAVVKRAQPADVNELTGV
SASPGLAVGKIVQFRQQVIDVKEAGESPQRERVRLEAAHHEARQNIEALKAKLTDPSKAQ
ILDAHLELLEDPDLNGMAIGSISEGKSAGFAWRAAFEQQAATLEKLDNPLLRERAGDVRD
VGRRVLALLAGMQQAQIDVPAGSILIAEELSPSDTASLDRTKVLGFCTTTGGATSHVAIL
ARSLGIPAICGIDEDALQLADGTLVVLDGSHGSLRRNPSAGEVEKARERISRQAEKREEE
KLAASKLAMTADGHRVEVVANIRNAKEARDAVAAGAEGVGLLRSEFLFDDRDTAPSEDEQ
ASEYCAVAEALGRERPLVIRTLDAGGDKPLSYMPLPKEDNPFLGLRGVRVSLDRPDIFRT
QLRAILRAAPIGNLHVMFPMVAAIEEVLAAKKILLEEAGDRANSIKVGVMIEVPAAALIA
EPLAREVDFFSIGTNDLTQYTLAMDRGHPKLARQADALHPAVLRLIGMTVEGAHKHGKWV
GVCGGIASDAIAVPVLVGLGVDELSVSVPAVGSIKAQLARLTMAQARQLAAEVVRLGTAA
EVRALLAPFAE