Protein Info for H281DRAFT_01660 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 4-hydroxyphenylacetate 3-monooxygenase reductase component (EC 1.14.13.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 181 TIGR02296: 4-hydroxyphenylacetate 3-monooxygenase, reductase component" amino acids 19 to 171 (153 residues), 221.3 bits, see alignment E=2.9e-70 PF01613: Flavin_Reduct" amino acids 23 to 171 (149 residues), 122.5 bits, see alignment E=8.1e-40

Best Hits

Swiss-Prot: 60% identical to HPAC_PSEAE: 4-hydroxyphenylacetate 3-monooxygenase reductase component (hpaC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 88% identity to bgf:BC1003_3889)

MetaCyc: 57% identical to HpaC (Escherichia coli W)
RXN-12446 [EC: 1.5.1.36]

Predicted SEED Role

"4-hydroxyphenylacetate 3-monooxygenase, reductase component (EC 1.6.8.-)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic Amin Catabolism (EC 1.6.8.-)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.36 or 1.6.8.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (181 amino acids)

>H281DRAFT_01660 4-hydroxyphenylacetate 3-monooxygenase reductase component (EC 1.14.13.3) (Paraburkholderia bryophila 376MFSha3.1)
MSAQPTPVQSQIDPLRQQFRQAMAHLGAAVNVITTAGAHGRCGITASAVCSVTDTPPTLL
VCLNRSSAMHAVFDGNRHVCINVLPANLEDMARHFAGMTGLSMEERFQLPEWYEGLNGVP
VLRGALASLQGRIVEAKDVGSHAVLFVETTEIRVRSDGDSLIYFDRNFHRVPRAWKGATG
R