Protein Info for H281DRAFT_01653 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Phenylpropionate dioxygenase, large terminal subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 45 to 126 (82 residues), 73.4 bits, E=1.1e-24
• PF00848: Ring_hydroxyl_A
  • amino acids 191 to 425 (235 residues), 65.9 bits, E=4.9e-22

Best Hits

KEGG orthology group: None (inferred from 96% identity to bgf:BC1003_3881)

Predicted SEED Role

"Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)" in subsystem Benzoate degradation (EC 1.14.12.10)

MetaCyc Pathways

• meta cleavage pathway of aromatic compounds (9/10 steps found)
• benzoate degradation I (aerobic) (2/2 steps found)
• mandelate degradation to acetyl-CoA (13/18 steps found)
• toluene degradation IV (aerobic) (via catechol) (9/13 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (22/35 steps found)
• superpathway of aerobic toluene degradation (16/30 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (18/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Fluorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.10

Use Curated BLAST to search for 1.14.12.10

Protein Sequence (427 amino acids)

>H281DRAFT_01653 Phenylpropionate dioxygenase, large terminal subunit (Paraburkholderia bryophila 376MFSha3.1)
MSTPTYQTIDTSALAKRAESDRIAPSLYYDPQLFEEELQRIFYRTWVWVAHDSELPKPGD
FVTTTIGRQPVIVVRDKTGAVNVLQNRCRHRGATVCEEHKGNAKGFTCPYHSWSYALDGT
LRALPYGDGYEGVCEKGDLPLKKLRVGVYQGLIFASFNEQIESLEDFLGGAKPWIDLFMK
QGAGYPIKANGEHKFKFKGNWKIQLENTTDLYHFPVVHKSWMKSIDDETAAVITSFMTSE
DAFCRSLGNGHSLAVLVPEIVDLDRDDGAPLPDRFNELAAQLSQKHTPEEVRRIVRSLMG
VGFNLNLFPNLALSMAFFRVLRPISAEETEIRHVALAMDGGPDEANRVRLRIHEHFQGPF
GFGSPDDAEAWERVQRGSYAGPDVPILVNRGLNRETAAPNGEKTAHATDETGMREAYRQW
RAMMEQA