Protein Info for H281DRAFT_01638 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 877 TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component" amino acids 27 to 873 (847 residues), 788.3 bits, see alignment E=4.9e-241 PF00676: E1_dh" amino acids 155 to 429 (275 residues), 122.2 bits, see alignment E=3.4e-39 PF02779: Transket_pyr" amino acids 525 to 725 (201 residues), 145.8 bits, see alignment E=1.6e-46 PF16870: OxoGdeHyase_C" amino acids 740 to 874 (135 residues), 122.5 bits, see alignment E=2e-39

Best Hits

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 84% identity to bug:BC1001_4352)

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.2

Use Curated BLAST to search for 1.2.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (877 amino acids)

>H281DRAFT_01638 2-oxoglutarate dehydrogenase E1 component (Paraburkholderia bryophila 376MFSha3.1)
MSHVSSESIVLSRSPASAVIEPAGDLSPVARTLQVRRFIDAFRQHGHRQASLDPLRLTPL
PDVPELDPVFHGLDPAQPIGAENPLLPPSAAVHELERQLRRVYCGAIGLDLTGIRDPQRR
ARLSAMLKSEQLAPLPGREQRRALLRRLISAEMWERHVAAVFEHAKRFSLEGCESLVPLL
EAIVDEAAAQGVQQVFAGMPHRGRLNVLVNVMGFDPAKLVDCLDPNSDVAATQRDLPYHL
GGSTVRQTPHGDTAIFLAHNPSHLQSVYPVVSGMARAYQDEHRGFGCLPVVVHGDAAFAG
QGIVMETLNMTRKDGYALGGTVHVIVNNQIGFTTPNRMDTEGHSYCTDVARMIDAPVIRV
NAERPDEVLRAAKLALQYRMEYEADVVIDLIGYRRLGHSEHDIPALTQPSQQAAISAHQT
VTELYHAAIGEETPLEMLRADVLKDLLTPPTQTLSQQRADRTSGAGPANPAKPARRTKRT
RPLSLARLQSLTAALTRTPPGLRLHDFVRDITAKWQRSVEAETATVDWCFAENLAYASLL
DDGHSVRISGMDVGRGTFMHRHAVWHSQGAISGATSGGTEPYVPLQNLGVHQGEFDIINS
PLTEEAVLGFEYGYSVQSTSHLAIWEAQFGDFVNGAQVIIDQYIAAGEYKWDQPSGLTML
LPHGHEGVGPEHSSAYLSRFLQLCAGGNMKVVMPSTSSQWFHMLREQALSVEPKPLVVMS
PKGQLHANERSHAPLGKLIDGAFAPVLQDSGAALPADVTRVVLCSGKFFYDLQAARDASG
DTQTALVRIEQLYPFPTSELALALAAYPNLAEVVWAQEEEKKHGAWHFVRDDIEGAVPAN
CRVFDMCRRESPSGADSSIRAHRVEQQRLVAAALART