Protein Info for H281DRAFT_01373 in Paraburkholderia bryophila 376MFSha3.1

Annotation: protocatechuate 4,5-dioxygenase beta subunit (EC 1.13.11.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02900: LigB
  • amino acids 8 to 272 (265 residues), 255 bits, E=3.6e-80

Best Hits

Swiss-Prot: 46% identical to PCYB_SPHSK: Protocatechuate 4,5-dioxygenase beta chain (ligB) from Sphingobium sp. (strain NBRC 103272 / SYK-6)

KEGG orthology group: K04101, protocatechuate 4,5-dioxygenase, beta chain [EC: 1.13.11.8] (inferred from 96% identity to bgf:BC1003_5350)

MetaCyc: 48% identical to protocatechuate 4,5-dioxygenase beta subunit (Comamonas testosteroni)
Protocatechuate 4,5-dioxygenase. [EC: 1.13.11.8]; 1.13.11.8 [EC: 1.13.11.8]; GALLATE-DIOXYGENASE-RXN [EC: 1.13.11.8, 1.13.11.57]; 1.13.11.- [EC: 1.13.11.8, 1.13.11.57]

Predicted SEED Role

"Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 1.13.11.3)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• gallate degradation I (4/4 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• methylgallate degradation (5/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (6/8 steps found)
• 3,4-dichlorobenzoate degradation (3/4 steps found)
• 4-sulfocatechol degradation (3/4 steps found)
• superpathway of vanillin and vanillate degradation (7/10 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• 3-chlorobenzoate degradation II (via protocatechuate) (3/6 steps found)
• syringate degradation (7/12 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (22/35 steps found)
• superpathway of aerobic toluene degradation (16/30 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 1.13.11.3, 1.13.11.8

Use Curated BLAST to search for 1.13.11.3 or 1.13.11.57 or 1.13.11.8

Sequence Analysis Tools

See A0A2Z5MPT4 at UniProt or InterPro

Protein Sequence (281 amino acids)

>H281DRAFT_01373 protocatechuate 4,5-dioxygenase beta subunit (EC 1.13.11.8) (Paraburkholderia bryophila 376MFSha3.1)
MARIIGGIGTSHVPTIGMAYDKGKQNDPAWAPLFKGYEPVANWLAERKPDAMVMFYNDHA
NSFFFDCYPTFALGVSGNHEFADEGAGKRPLPDIAGHPDLAIHIAEQLVNDEFDLTIFQD
RPLDHGCNSPLSLMLPHAGGWPLALVPVQVNVLQYPLPTANRCYRLGQALRRAIESFEED
LDVVVVGTGGLSHQVHGERSGFNNTEWDMEFLDLIVHEPQRLAAMKHVDYVRLGGAESVE
TIMWLAMRGALGPKIRELHRNYYLATSTAMAVTIYEDEVAQ