Protein Info for H281DRAFT_01343 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 3-methylcrotonoyl-CoA carboxylase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 152.3 bits, see alignment E=2.2e-48 PF02786: CPSase_L_D2" amino acids 116 to 322 (207 residues), 246.5 bits, see alignment E=7.1e-77 PF07478: Dala_Dala_lig_C" amino acids 139 to 291 (153 residues), 33.4 bits, see alignment E=1.2e-11 PF02222: ATP-grasp" amino acids 142 to 292 (151 residues), 37.4 bits, see alignment E=7.1e-13 PF02785: Biotin_carb_C" amino acids 336 to 456 (121 residues), 124.5 bits, see alignment E=7e-40 PF00364: Biotin_lipoyl" amino acids 609 to 673 (65 residues), 62.1 bits, see alignment E=1.3e-20

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 94% identity to bug:BC1001_3992)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (677 amino acids)

>H281DRAFT_01343 3-methylcrotonoyl-CoA carboxylase, alpha subunit (Paraburkholderia bryophila 376MFSha3.1)
MFNKILIANRGEIACRVAATCKRLGIASVAVYSDADADAKHVSACDEAVHIGGATAAESY
LRVARIIEAARATGAQAVHPGYGFLSENEDFAHACEAAGIVFIGPPVEAIAAMGSKAAAK
ALMHAAAVPLVPGYHGDDQNPELLHREADAIGYPVLLKASAGGGGKGMRVVERSEDFLAA
LASCKREAASSFGNDRVLIEKYLTRPRHVEVQVFADRHGGAVYLFDRDCSVQRRHQKVLE
EAPAPGLSAQIKREMGEAAVAAARAVNYVGAGTVEFIMTATGDFYFMEMNTRLQVEHPVT
EMITGQDLVEWQLRVAAGQPLPLTQDQLKIDGHAIEARIYAEHPARGFLPSTGTLKHLRM
PEGVEFAIHAGAGESRKAPVRIDSGVREGDTITPFYDPMIAKLIVHGATREEALARMSRA
LHACEVVGPHTNVEFLQRIVESEPFATGDLDTGLIERHHEALFAPVKKPFKEALALACAA
LLTREGGTAHGASPWDALSHWRLNSGFTQTLGWLAIESSGETGSPFTVAFARDGATQTLE
HDGVREAFSWSSGAGLHEYRATIGDARATGRVFIDGDTFHVFCLGHALAFEWQNLLAHAA
DAEHGEGRLTAPMPGKVIAVLVEPGAVVEKGTPLIVMEAMKMEHTIGAPAAGTVSEVLYA
VGDQVADGAQLLVLDVQ