Protein Info for H281DRAFT_01317 in Paraburkholderia bryophila 376MFSha3.1

Annotation: ribulose 1,5-bisphosphate carboxylase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 96% identical to RBL_PARXL: Ribulose bisphosphate carboxylase large chain (cbbL) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K01601, ribulose-bisphosphate carboxylase large chain [EC: 4.1.1.39] (inferred from 97% identity to bgf:BC1003_5439)

MetaCyc: 60% identical to ribulose-bisphosphate carboxylase/oxygenase large subunit (Arabidopsis thaliana col)
Ribulose-bisphosphate carboxylase. [EC: 4.1.1.39]; 1.13.11.- [EC: 4.1.1.39]

Predicted SEED Role

"Ribulose bisphosphate carboxylase large chain (EC 4.1.1.39)" in subsystem CO2 uptake, carboxysome or Calvin-Benson cycle or Carboxysome or Photorespiration (oxidative C2 cycle) (EC 4.1.1.39)

MetaCyc Pathways

Rubisco shunt (10/10 steps found)
Calvin-Benson-Bassham cycle (11/13 steps found)
ethene biosynthesis V (engineered) (19/25 steps found)
1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
oxygenic photosynthesis (12/17 steps found)
photorespiration I (6/9 steps found)
photorespiration III (6/9 steps found)
photorespiration II (6/10 steps found)
nucleoside and nucleotide degradation (archaea) (1/10 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.39

Use Curated BLAST to search for 4.1.1.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5ML86 at UniProt or InterPro

Protein Sequence (499 amino acids)

>H281DRAFT_01317 ribulose 1,5-bisphosphate carboxylase large subunit (Paraburkholderia bryophila 376MFSha3.1)
MNDFSQPVIESLHKPRDAGNARERYAAGVMKYREMGYWQPDYTPKDTDVIALFRITPQPG
VEPEEAAAAVAGESSTATWTVVWTDRLTACDMYRAKAYRVDPVPASNAAEPQYFAYIAYE
LDLFEEGSIANLTASIIGNVFGFKPLKALRLEDMRIPVAYLKTFQGPPTGIVVERERLDK
YGRPLLGATVKPKLGLSGKNYGRVVYEGLRGGLDFLKDDENINSQAFMHWRDRFLFSMEA
VNRAQAETGEVKGHYLNVTAGTMEEMYERAEFAKELGSCIVMIDLVIGWTAIQSMSRWAR
RNDMILHLHRAGHSTYTRQRNHGISFRVIAKWLRMAGVDHAHAGTAVGKLEGDPLTVQGF
YNVCREARNEVDLSRGIFFEQPWAGLRKVMPVASGGIHAGQMHQLLDLFGDDAILQFGGG
TIGHPGGIQAGAVANRVALEAMVKARNEGRDIRHEGPDILEAAARWCGPLKQALDTWRDV
SFNYASTDSPDFAATPTAA