Protein Info for H281DRAFT_01296 in Paraburkholderia bryophila 376MFSha3.1

Annotation: PQQ-dependent dehydrogenase, methanol/ethanol family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 494 to 512 (19 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 23 to 559 (537 residues), 722.9 bits, see alignment E=1.1e-221 PF13360: PQQ_2" amino acids 67 to 221 (155 residues), 48.9 bits, see alignment E=1e-16 amino acids 477 to 539 (63 residues), 21.6 bits, see alignment E=2.3e-08 PF01011: PQQ" amino acids 140 to 169 (30 residues), 26.9 bits, see alignment (E = 4.3e-10) amino acids 506 to 536 (31 residues), 20.1 bits, see alignment (E = 6.2e-08) PF13570: PQQ_3" amino acids 483 to 521 (39 residues), 20.5 bits, see alignment 7.7e-08

Best Hits

Swiss-Prot: 63% identical to XOXF_PARDE: Putative dehydrogenase XoxF (xoxF) from Paracoccus denitrificans

KEGG orthology group: None (inferred from 98% identity to bug:BC1001_3943)

Predicted SEED Role

"Methanol dehydrogenase large subunit protein (EC 1.1.99.8)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MJ84 at UniProt or InterPro

Protein Sequence (605 amino acids)

>H281DRAFT_01296 PQQ-dependent dehydrogenase, methanol/ethanol family (Paraburkholderia bryophila 376MFSha3.1)
MNLRTTVLGLAILASAAMSSIVAQADSQLDGLMKSPSNWAAQAGDYANHRYSPLKQINEN
NVGKLQVAWTMSTGVLRGHEGAPLVIGDTMYIHSPFPNKVIAINLKDQTFLWQYQPKQDD
SVVSVMCCDTVNRGLAYGDGKIFLQQADTKLVALNAKTGDVVWTAQNGNSKAGETNTNAP
HVFGDKVLTGISGGEFGVRGRLIAYDIKTGKPAWTAYSTGPDKDMLMDPDKTMTYADGKM
VPVGADSSLKSWKGDQWKLGGGTTWGWYAWDPKLNLVYYGTGNPGTWNPTQRPGDNKWSM
SIMARDLNTGMAKWVYQMTPHDEWDYDGVNEMILSDLTIDGKKVPAIVHFDRNGFGYTLN
RESGQLLVAQKFDPAVNWADSVDLKSGKPIRNAAYSTQAAGSDHNVKGICPAALGSKDQQ
PAAYDPNSSLFLVPTNHVCMDYEPFDVDYVSGQPYVGATLSMYPGPNENNAMGNFIAWDA
AKGKIVWSKPEKFSVWSGALATAGGIAFYGTLEGYLKAVRIKDGKELWRFKTPSGIIGNV
FTYQYQGKQYVGVYSGIGGWAGIGMAAGLEKSTEGLGAVGGYRELAKYTALGGTLFVFAI
PGGNS