Protein Info for H281DRAFT_01278 in Paraburkholderia bryophila 376MFSha3.1

Annotation: trans-feruloyl-CoA synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 629 PF00501: AMP-binding" amino acids 60 to 435 (376 residues), 180 bits, see alignment E=3.3e-57

Best Hits

KEGG orthology group: K12508, feruloyl-CoA synthase [EC: 6.2.1.34] (inferred from 89% identity to bug:BC1001_3919)

MetaCyc: 52% identical to 4-coumarate-CoA ligase (Rhodopseudomonas palustris CGA009)
4-coumarate--CoA ligase. [EC: 6.2.1.12]

Predicted SEED Role

"Trans-feruloyl-CoA synthase (EC 6.2.1.34)" (EC 6.2.1.34)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.12 or 6.2.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MIR3 at UniProt or InterPro

Protein Sequence (629 amino acids)

>H281DRAFT_01278 trans-feruloyl-CoA synthase (Paraburkholderia bryophila 376MFSha3.1)
VNLQPTQSSVDRETPVRYREVRIGSAPARVRRDGDVWYMESAAMPGNYPQRLTDRLASGA
RAHPDRWLVARRGANGEWQGISYAQMLQRARAIGQALLERNLSAERPVAILSGNDLEHMM
IALGAMWAGVPYAPISPAYSLVSSDFGKLRHTLDVLTPGLVFATDASAFAAAIDATVAAD
VEVVTSTGCCARPVTKLSALLDTTPVTVDAAHEAIDPDSIAKFLFTSGSTTLPKAVPTTH
RMLCSNQQMLRETFPEFADEPPVLVDWLPWNHTFGGSHNAGIALYNGGTLYIDDGKPVGK
KFEETLRNLREIAPTIYFNVPKGWEELTSALETDAKLRETFFSRVKVYFFAGAGLSQAAW
DRLQQVTERFCGERIRIMAGLGMTETSPSCLFTTLPAKAAGYVGVPAPGCEVKLVPIGDK
LEARFRGPHVMRGYWRMDGAATGKSFDDEGYYCSGDALKFVDPQQPELGLMFDGRIAEDF
KLSSGTFVSVGPMRARVISAGAPYVQDVVVSGLNCDDVGLLVFPRLDDCRRLAQLPSSAS
ASEVVSAPAVRQFFVELLDRLNRESTGGATTIARMRLLDVAPSLDLGEITDKGSINQRAV
LSHRAELVQALHDPIQRDPAVIYAPHVRT