Protein Info for H281DRAFT_01203 in Paraburkholderia bryophila 376MFSha3.1

Annotation: acetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 47 to 387 (341 residues), 207.2 bits, E=3.5e-65
• PF13193: AMP-binding_C
  • amino acids 457 to 534 (78 residues), 57.9 bits, E=1.6e-19

Best Hits

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 95% identity to bug:BC1001_3824)

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• chitin deacetylation (3/4 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (4/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1

Sequence Analysis Tools

See A0A2Z5MK48 at UniProt or InterPro

Protein Sequence (567 amino acids)

>H281DRAFT_01203 acetyl-CoA synthetase (Paraburkholderia bryophila 376MFSha3.1)
MTAAKAFFDARDLLLRHRTDYERAYREFAWPALEQFNWALDYFDVIARDNDNTALWIVDD
PASEGLRLSYAQMSERSARMANFLRSVGVKRGDRVLLMLPNRVELWDVMLAAMKLGAIVL
PATTQLSADDVRDRVQIGGANFVVVDSAELSKFDSLDVPLTRLSVGTPRAGWTDLAAAYD
ASPQFTPDGVTRAADPLLLYFTSGTTSKPKLVEHTHQSYPVGHLSTMYWIGLQPGDIHWN
ISSPGWAKHAWSCFFAPWNAQACVFVFNFARFVPKDTLDVLVRFNVTTVCAPPTVWRMLV
QEHLTDYPVKLREIVGAGEPLNPEIIERVKHAWGITIRDGFGQTETTCQIGNSPGQPVVA
GSMGRPLPGYRIELLDADDQPVTEGEIALPIGPDGKQRPLGLMTGYANSEQATAQAMRNG
FYRTSDVALRREDGYYVYVGRADDVFKSSDYRLSPFELESVLIEHEAIGEAAVVPSADAL
RLSVPKAFVTVRHGYQAGPELARAVFAFSREKLAPYKRIRRLQFSELPKTISGKIRRVEL
RRREMERQAEPARLPDEYWEEDFPDLR