Protein Info for H281DRAFT_01081 in Paraburkholderia bryophila 376MFSha3.1

Annotation: L-glutamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00120: Gln-synt_C
  • amino acids 109 to 438 (330 residues), 358.2 bits, E=2.3e-111

Best Hits

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 96% identity to bug:BC1001_1611)

Predicted SEED Role

"Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)" (EC 6.3.1.11)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• ammonia assimilation cycle II (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• superpathway of L-arginine and L-ornithine degradation (9/13 steps found)
• superpathway of ornithine degradation (5/8 steps found)
• putrescine degradation II (2/4 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (7/11 steps found)
• L-aspartate degradation II (aerobic) (1/3 steps found)
• L-aspartate degradation III (anaerobic) (1/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.2

Use Curated BLAST to search for 6.3.1.11 or 6.3.1.2

Sequence Analysis Tools

See A0A2Z5MH23 at UniProt or InterPro

Protein Sequence (444 amino acids)

>H281DRAFT_01081 L-glutamine synthetase (Paraburkholderia bryophila 376MFSha3.1)
MHDIDEFLKKNHVTEIEAIIPDMAGIARGKIIPRSKFESGESMRLPQAVMIQTVTGDYPD
GGTLTGVTDPDMVCVPDSSTIRMIPWAVDPTAQVIHDCVHFDGTPVAISPRRVLRRVLEL
YKAKGWKPVIAPELEFYLVDMNKDPDLPLQPPIGRTGRAETGRQAYSIEAVNEFDPLFED
IYEYCDVQELEVDTLIHEVGAAQMEINFMHGDPLKLADSVFLFKRTVREAALRHKMYATF
MAKPMEGEPGSAMHMHQSLVDGRTGHNLFTGADGKPTSLFTSYIAGLQKYTPALMPIFAP
YINSYRRLSRFMAAPINVAWGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLAIAATL
AAGYLGMTQKLDATEPLLSDGYELPYQLPRNLEEGLTLMGACEPIAEVLGDKFVQAYLAL
KETEYEAFFRVISSWERRHLLLHV