Protein Info for H281DRAFT_01038 in Paraburkholderia bryophila 376MFSha3.1

Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01642: MM_CoA_mutase
  • amino acids 10 to 520 (511 residues), 677.7 bits, E=1e-207
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 10 to 524 (515 residues), 694.9 bits, E=5.4e-213
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 560 to 681 (122 residues), 115.4 bits, E=1.8e-37
• PF02310: B12-binding
  • amino acids 563 to 672 (110 residues), 65.1 bits, E=5.4e-22

Best Hits

Swiss-Prot: 47% identical to MUTA_MOUSE: Methylmalonyl-CoA mutase, mitochondrial (Mmut) from Mus musculus

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 91% identity to bph:Bphy_4569)

MetaCyc: 48% identical to Methylmalonyl-CoA mutase, mitochondrial (Homo sapiens)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
• propanoyl CoA degradation I (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• methylaspartate cycle (12/19 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• superpathway of the 3-hydroxypropanoate cycle (10/18 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.2

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (703 amino acids)

>H281DRAFT_01038 methylmalonyl-CoA mutase (Paraburkholderia bryophila 376MFSha3.1)
MNTQRRRRSDRVKLKRVYTRADIEGIAHIDSMPGEAPFVRGPFASMYTQKPWTIRQYAGY
AQASDTNLAFRTALAEGAQGLSVAFDLPTQRGYDSDDPAVSADVGMTGVAIDTVEDMTRL
FEDIALDRVSVSMTMNGAVLPVLAAFIVAAEESGVGASQLRGTIQNDILKEFMVRNTSIF
APEPSLRIAADVVEYLGKNVPRFNALSVSGYHFQEAGADPVLELALTMANARKYITALAG
RGMQADDVCQRMSFFFGVGMDFYVEIAKLRAARILWADIASASGATSDRARALRMHCQTS
GWSLTAQKPMNNVVRTTVEALAAVFGGTQSLHTNGYDEALSLPCADASRLARDTQLVLQH
ETGVCDVVDPWAGSYMMEKFTAEICQKALTVMAEIERRGGVVDVVESGWVRDQIHRSALN
IQAEIESRKRTVVGVNDFMTGCDEDLGEPQTVDGRRVRVLQTQRLAHIKARRDPTRVRNT
LDALTRAARGRDGNLLELTVACMRARATVGECTQALEAVWPRYQIGLPLLRDHYGANLES
DKDWQDTCRAVADATKRLGRAPRVLIAKLGQDGHDRGARIIAAALSDAGFAVFTGSMFAS
PSDTVQLAEGERVDVIGVSSLAGAQNDLITALHDQLTVRGLRIPIVVGGIIDQASQLVLR
RSGVAACFPAGTPIKKIVTLLAAIAVASAEKCVEGGHPLHESA