Protein Info for H281DRAFT_00963 in Paraburkholderia bryophila 376MFSha3.1

Annotation: malate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 7 to 722 (716 residues), 1210.7 bits, E=0
• PF20656: MS_N
  • amino acids 18 to 76 (59 residues), 64.7 bits, 8.9e-22
• PF20658: MSG_insertion
  • amino acids 160 to 234 (75 residues), 120.1 bits, E=7.2e-39
• PF01274: MS_TIM-barrel
  • amino acids 337 to 569 (233 residues), 230.9 bits, E=2.5e-72
• PF20659: MS_C
  • amino acids 593 to 688 (96 residues), 86.9 bits, E=2.9e-28

Best Hits

Swiss-Prot: 94% identical to MASZ_PARXL: Malate synthase G (glcB) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 94% identity to bxe:Bxe_A2223)

MetaCyc: 66% identical to malate synthase G (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• glyoxylate cycle (6/6 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• L-arabinose degradation IV (6/8 steps found)
• chitin deacetylation (3/4 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• D-xylose degradation IV (4/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)
• superpathway of pentose and pentitol degradation (21/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.9

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See A0A2Z5MAF3 at UniProt or InterPro

Protein Sequence (723 amino acids)

>H281DRAFT_00963 malate synthase (Paraburkholderia bryophila 376MFSha3.1)
MTQMNPRGGLQVAANLDQFVETEALPGTGIDSAAFWSGFDALVHELAPKNHALLAERDRL
QTELDNWHRANPGPVRDLRAYRAFLEGIGYIVPAPASVKATTDQVDTEIAEQAGPQLVVP
LSNQRYALNAANARWGSLYDALYGTDAIPETNGADRQKTFNPVRGAAVIAYARKFLDEAA
PLANGSHADATRYSVEGGKLVVALKNGTSELKTPAQFVGYQGDASAPSAVLLKHNGLHFE
IQIDANDSIGKTDAAHVKDVVVEAAVSTIIDCEDSVAAVDADDKVQLYRNWLGLMNGDLT
EEVTKNGKTFTRRLNDDRVYVAANGTAPVVLHGRSLLFIRNVGHLMTNPAVLTKDGAEIP
EGILDAVMTTLCALHDRKHKLNSRTGSIYIVKPKMHGPAEVAFASELFARVEDLLKLPRN
TIKMGIMDEERRTSVNLLACIAQASERVAFINTGFLDRTGDEMHSAMEAGPMMRKGDMKS
SAWIAAYERSNVLVGLHAGLRGRSQIGKGMWAMPDLMHAMLEQKIAHPKAGANTAWVPSP
TAATLHALHYHQIDVQAVQQQLERTDYASVRDELLDGLLTIPVVETANWSADEIRAELDN
NAQGILGYVVRWIDQGVGCSKVPDIHNIGLMEDRATLRISSQHIANWLYHGVVTREQVEE
TFKRMAQVVDQQNAGDPNYRPMAPSFDTIAFKAAQALVFEGRQQPSGYTEPLLHKFRLQV
KNG