Protein Info for H281DRAFT_00743 in Paraburkholderia bryophila 376MFSha3.1

Annotation: dihydroorotate oxidase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 1 to 324 (324 residues), 427 bits, E=2.3e-132
• PF01180: DHO_dh
  • amino acids 36 to 324 (289 residues), 280.1 bits, E=2e-87
• PF03060: NMO
  • amino acids 238 to 311 (74 residues), 29.5 bits, E=5.3e-11

Best Hits

Swiss-Prot: 97% identical to PYRD_PARXL: Dihydroorotate dehydrogenase (quinone) (pyrD) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 97% identity to bgf:BC1003_1451)

MetaCyc: 56% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Sequence Analysis Tools

See A0A2Z5M9X4 at UniProt or InterPro

Protein Sequence (325 amino acids)

>H281DRAFT_00743 dihydroorotate oxidase A (Paraburkholderia bryophila 376MFSha3.1)
MDAEDAHHLTLRVLGAAGRTGLAGALAARVPDAPRTVMGLTFRNPVGLAAGLDKDGASID
GLAALGFGFIEVGTVTPRAQPGNPRPRMFRLPEANAVINRMGFNNAGVDQFVKNVQSARY
RGILGLNIGKNADTPIERAAEDYLYCLERVYPFASYVTVNISSPNTKNLRQLQGAGELDA
LLAALKDKQQRLADMHGKLVPLALKIAPDLDDEQIKSIADTLLRHRFEGVIATNTTLSRT
AVAGMQYGDEAGGLSGKPVFDASNEVIRKLRAEVGEAVPIIGVGGIFSGDDARAKLAAGA
SLVQLYTGFIYRGPALVAECVQALR