Protein Info for H281DRAFT_00649 in Paraburkholderia bryophila 376MFSha3.1
Annotation: 4-hydroxythreonine-4-phosphate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to PDXA2_CUPNH: D-threonate 4-phosphate dehydrogenase (pdxA2) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)
KEGG orthology group: K00097, 4-hydroxythreonine-4-phosphate dehydrogenase [EC: 1.1.1.262] (inferred from 96% identity to bgf:BC1003_0576)MetaCyc: 48% identical to 4-phospho-D-tetronate 3-dehydrogenase (Salmonella enterica enterica serovar Typhimurium str. LT2)
RXN-18600 [EC: 1.1.1.408]
Predicted SEED Role
"4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.1.1.262)
MetaCyc Pathways
- superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
- pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
- L-threonate degradation (2/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.262
Use Curated BLAST to search for 1.1.1.262 or 1.1.1.408
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2Z5MAA0 at UniProt or InterPro
Protein Sequence (333 amino acids)
>H281DRAFT_00649 4-hydroxythreonine-4-phosphate dehydrogenase (Paraburkholderia bryophila 376MFSha3.1) MSNYLPVIGITMGDAAGVGPEIIVKSLAHDIVYKQCRPLVIGDARRLERANEIVGGNARI RRIKEAAEARYEPGTIDCIDLDLIPADMPFGQLSAVAGDAAYQFIARAVQLAEAKQIDAI CTAPLNKEALHAGGHKFPGHTEMLAYLTGVDEVSMMLVAPQLRVIHVTTHIGIIDAIRKI EPGLVQRTIERGNATLVKAGIAKPRIGVCGINPHAGENGLFGYGEEEEKIVPAVKVLQER GLDVTGPLPADTLFFRAGRGDFDLVVAMYHDQGHGPVKVLGLEAGVNVTVGLEVIRTSVD HGTAFDIAGKGIADERSLLEALRQGAELATRRG