Protein Info for H281DRAFT_00644 in Paraburkholderia bryophila 376MFSha3.1

Updated annotation (from data): 2-deoxy-D-ribonate 3-dehydrogenase
Rationale: Important for utilization of deoxyribonate and deoxyribose. Similar to the deoxyribonate dehydrogenase of P. simiae (PS417_07245).
Original annotation: NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 263 signal peptide" amino acids 12 to 13 (2 residues), see Phobius details amino acids 30 to 30 (1 residues), see Phobius details transmembrane" amino acids 14 to 29 (16 residues), see Phobius details amino acids 31 to 32 (2 residues), see Phobius details PF00106: adh_short" amino acids 15 to 197 (183 residues), 158.1 bits, see alignment E=4e-50 PF08659: KR" amino acids 19 to 172 (154 residues), 30.2 bits, see alignment E=8.4e-11 PF13561: adh_short_C2" amino acids 20 to 258 (239 residues), 192.7 bits, see alignment E=1.5e-60 PF08643: DUF1776" amino acids 98 to 187 (90 residues), 28.3 bits, see alignment E=2.3e-10

Best Hits

KEGG orthology group: None (inferred from 77% identity to bpy:Bphyt_0980)

MetaCyc: 59% identical to 2-deoxy-D-ribonate dehydrogenase (Pseudomonas simiae)
1.1.1.M55 [EC: 1.1.1.M55]

Predicted SEED Role

"D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30)" in subsystem Polyhydroxybutyrate metabolism (EC 1.1.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.30

Use Curated BLAST to search for 1.1.1.30 or 1.1.1.M55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MC75 at UniProt or InterPro

Protein Sequence (263 amino acids)

>H281DRAFT_00644 2-deoxy-D-ribonate 3-dehydrogenase (Paraburkholderia bryophila 376MFSha3.1)
MSIIDLLKPRPGLRVFVSAGAAGIGLAIAEAFIEAQAEVYICDVNQAAIDEATSRFPKLH
AGIADVSKQAQVDQIIDDARRKLGGLDVLVNNAGIAGPTGAVEELDPAQWESTVSTNLNS
QFYFLRKAVPVLKETSDCASIIAMSSVAGRLGYPFRTPYASTKWAIVGLVKSLAAELGPS
NVRVNAILPGVVEGERMDRVISARADALGIPFNAMREEYLKKISLRRMVTVDDIAAMALF
LASPAGSNVTGQAISVDGNVEYL