Protein Info for H281DRAFT_00580 in Paraburkholderia bryophila 376MFSha3.1

Annotation: isocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01346: isocitrate lyase
  • amino acids 9 to 253 (245 residues), 372.5 bits, E=1.5e-115
• PF00463: ICL
  • amino acids 13 to 253 (241 residues), 201.5 bits, E=2.2e-63
  • amino acids 255 to 426 (172 residues), 153.9 bits, E=6e-49
• PF13714: PEP_mutase
  • amino acids 79 to 278 (200 residues), 52.4 bits, E=5.9e-18

Best Hits

Swiss-Prot: 70% identical to ACEA_BACHD: Isocitrate lyase (aceA) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: K01637, isocitrate lyase [EC: 4.1.3.1] (inferred from 99% identity to bgf:BC1003_1258)

MetaCyc: 69% identical to isocitrate lyase subunit (Mycobacterium tuberculosis H37Rv)
Isocitrate lyase. [EC: 4.1.3.1]

Predicted SEED Role

"Isocitrate lyase (EC 4.1.3.1)" in subsystem Serine-glyoxylate cycle (EC 4.1.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.1

Use Curated BLAST to search for 4.1.3.1

Sequence Analysis Tools

See A0A2Z5MAD0 at UniProt or InterPro

Protein Sequence (435 amino acids)

>H281DRAFT_00580 isocitrate lyase (Paraburkholderia bryophila 376MFSha3.1)
MTTREEQARQLQQQWETDPRWKGVKRSYTAEDVIRLRGSVQVEHTLAKRGAEKLWHSVNT
EPFVNSLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANVAGEMYPDQSLYPANSVPLVVK
RINNTLTRADQIQWSEGKNPGDEGYIDYFAPIVADAEAGFGGVLNAFELMKAMIEAGAAG
VHFEDQLASVKKCGHMGGKVLVPTRENVAKLTAARLAADVSGVSTVLLARTDAEAADLIT
SDVDENDKPFLTGERTVEGFYRTKPGLEQAISRGLAYAPYADMIWCETGKPDLEFAKKFA
DAIHKEYPDQLLSYNCSPSFNWKKNLDDATIAKFQRELGAMGYKFQFITLAGFHALNYSM
FNLAHGYARNQMTAFVEMQQAEFAAAEKGFTAVKHQREVGTGYFDAVTQTVEREASTTAL
HGSTEDEQFFDKKVA