Protein Info for H281DRAFT_00516 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase" amino acids 30 to 423 (394 residues), 388.9 bits, see alignment E=1.2e-120 PF04551: GcpE" amino acids 45 to 424 (380 residues), 379 bits, see alignment E=1.1e-117

Best Hits

Swiss-Prot: 91% identical to ISPG_BURCJ: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 98% identity to bpy:Bphyt_2541)

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (438 amino acids)

>H281DRAFT_00516 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (Paraburkholderia bryophila 376MFSha3.1)
MGFSMQSEAQSQSSSKIVSNEPVFGGQAARRKSHAVDVRWGGQLVTIGGDAPVRVQSMTN
TDTADAIGTAIQIKELAQAGSELVRITVNTPEAAAAVPAVREQLDRMGVSVPLVGDFHYN
GHLLLRDYPACAESLSKYRINPGNVGHGAKRDTQFAQMIEAAAKYDKPVRIGVNWGSLDQ
DLLAKMMDENAARATPWEAQSVMYEALIQSAIGSAERAVELGLSRNKIILSCKVSGVQDL
IAVYRELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGASR
TGEVIVGQEILQTMGLRSFTPMVIACPGCGRTTSTLFQELASQIQTYLRTQMPVWRDQYP
GVEKMHVAVMGCIVNGPGESKQANIGISLPGSGENPAAPVFIDGEKVKTLRGENIAQEFQ
QIVSDYVERRYGRAEALN