Protein Info for H281DRAFT_00486 in Paraburkholderia bryophila 376MFSha3.1

Annotation: lysyl-tRNA synthetase, class II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 TIGR00499: lysine--tRNA ligase" amino acids 44 to 534 (491 residues), 657.8 bits, see alignment E=5e-202 PF01336: tRNA_anti-codon" amino acids 96 to 173 (78 residues), 56.2 bits, see alignment E=2.7e-19 PF00152: tRNA-synt_2" amino acids 189 to 532 (344 residues), 323.3 bits, see alignment E=1.7e-100

Best Hits

Swiss-Prot: 96% identical to SYK_PARPJ: Lysine--tRNA ligase (lysS) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 96% identity to bxe:Bxe_A1561)

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MBQ8 at UniProt or InterPro

Protein Sequence (536 amino acids)

>H281DRAFT_00486 lysyl-tRNA synthetase, class II (Paraburkholderia bryophila 376MFSha3.1)
MRTTESGPPIADHEYQTIMNEPIQPNTAQPDVAQPSVVPEVDDNKIIAERREKLREVREQ
GIAYPNDFRPTHHAEDLQSQFEHTDKEALEANPLEVAIAGRMMLKRVMGKASFATVRDGS
GQIQFFITPADVGQQTYDAFKKWDMGDIVAARGVLFRTNKGELSVRCTELRLLSKSLRPL
PDKFHGLSDQEMKYRQRYVDLIVTPETRKTFVARTKAISSIRKFMADADFMEVETPMLHP
IPGGAAAKPFTTHHNALDMQMFLRIAPELYLKRLVVGGFERVFEINRNFRNEGVSVRHNP
EFTMIEFYAAYTDYKWVMDFTEQLIRQAAIDALGTATITYQGRELDLAKPFHRLTITQAI
QKYAPQYTNEQLADSAFLRTELKKFGVDASQPQFLNAGVGSLQLALFEETAESQLWEPTY
IIDYPIEVSPLARASDKVEGITERFELFITGREIANGFSELNDPEDQAARFKKQVDQKDA
GDEEAMFYDADYIRALEYGMPPAGGCGIGIDRLVMMLTDSPSIRDVILFPHLRRED