Protein Info for H281DRAFT_00303 in Paraburkholderia bryophila 376MFSha3.1

Annotation: formate-dependent phosphoribosylglycinamide formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01142: phosphoribosylglycinamide formyltransferase 2
  • amino acids 17 to 399 (383 residues), 586.8 bits, E=8.8e-181
• PF01370: Epimerase
  • amino acids 22 to 85 (64 residues), 24.1 bits, E=5.5e-09
• PF02786: CPSase_L_D2
  • amino acids 125 to 204 (80 residues), 26.5 bits, E=1.1e-09
• PF02222: ATP-grasp
  • amino acids 127 to 304 (178 residues), 164.8 bits, E=3.6e-52
• PF07478: Dala_Dala_lig_C
  • amino acids 129 to 294 (166 residues), 28.3 bits, E=3e-10
• PF21244: PurT_C
  • amino acids 330 to 398 (69 residues), 88.7 bits, E=3.5e-29

Best Hits

Swiss-Prot: 95% identical to PURT_PARXL: Formate-dependent phosphoribosylglycinamide formyltransferase (purT) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K08289, phosphoribosylglycinamide formyltransferase 2 [EC: 2.1.2.2] (inferred from 95% identity to bpy:Bphyt_1245)

MetaCyc: 62% identical to phosphoribosylglycinamide formyltransferase 2 (Escherichia coli K-12 substr. MG1655)
GARTRANSFORMYL2-RXN [EC: 6.3.1.21]; Acetate kinase. [EC: 6.3.1.21, 2.7.2.1, 2.7.2.15]

Predicted SEED Role

"Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• Bifidobacterium shunt (14/15 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• mixed acid fermentation (13/16 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)
• ethanolamine utilization (5/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• acetyl-CoA fermentation to butanoate (6/7 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• acetate and ATP formation from acetyl-CoA I (2/2 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• acetylene degradation (anaerobic) (4/5 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)
• pyruvate fermentation to acetate I (2/3 steps found)
• pyruvate fermentation to acetate IV (2/3 steps found)
• pyruvate fermentation to acetate VII (2/3 steps found)
• glycine degradation (reductive Stickland reaction) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (6/9 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (6/9 steps found)
• (S)-propane-1,2-diol degradation (3/5 steps found)
• pyruvate fermentation to acetate and lactate II (2/4 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• methanogenesis from acetate (2/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (4/9 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• superpathway of methanogenesis (5/21 steps found)
• superpathway of L-lysine degradation (11/43 steps found)

KEGG Metabolic Maps

• Nucleotide sugars metabolism
• One carbon pool by folate
• Propanoate metabolism
• Purine metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.2, 2.7.2.1

Use Curated BLAST to search for 2.1.2.- or 2.1.2.2 or 2.7.2.1 or 2.7.2.15 or 6.3.1.21

Sequence Analysis Tools

See A0A2Z5M8X3 at UniProt or InterPro

Protein Sequence (407 amino acids)

>H281DRAFT_00303 formate-dependent phosphoribosylglycinamide formyltransferase (Paraburkholderia bryophila 376MFSha3.1)
MQIGQRIGTPLSESATRVMLLGAGELGKEVIISLQRLGVEVIAVDRYPNAPGHQVAHRTH
VIDMTDGAALRALVEQERPHLIVPEIEAIATDALAAIESDGLAEVIPTARATQLTMNREG
IRRLAAEELGLPTSPYAFADSLDELRAGIAKVGYPCVVKPVMSSSGKGQSVLKSDADVEA
AWQYAMAGGRVNYGRVIVEGFIDFDYEITQLTVRAVDPSTGEVATYFCDPIGHVQVAGDY
VESWQPQPMNPLALERSREVAHKVTAALGGRGLFGVELFVRGDDVWFSEVSPRPHDTGLV
TLCSQRFSEFELHARAILGLPVDTSLRAPGASAVIYGGLDEAGIAFEGVAAALAVPNADL
RLFGKPESFVKRRMGVALATGGDIDEARSRAKEAAAAVRPVSGKSAQ