Protein Info for H281DRAFT_00297 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 PF19291: TREH_N" amino acids 17 to 189 (173 residues), 68.1 bits, see alignment E=6.8e-23 PF00723: Glyco_hydro_15" amino acids 239 to 597 (359 residues), 301.3 bits, see alignment E=1.2e-93

Best Hits

KEGG orthology group: None (inferred from 94% identity to bgf:BC1003_1022)

Predicted SEED Role

"Glucoamylase (EC 3.2.1.3)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.3

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M8V0 at UniProt or InterPro

Protein Sequence (640 amino acids)

>H281DRAFT_00297 Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family (Paraburkholderia bryophila 376MFSha3.1)
LRRTCYSNALIRKLMPAPIEDYALIGDGHTAALISREGSVDWLCWPRFDSGACFAALLGT
PDNGRWLICPDTDAPVKITRRYRGETLILETDFETPEGAVTLVDFMPPGNGWSEMVRIVV
GKRGTVRMKMELVLRFDYGFSIPWVQRLKNESGIRAIVGPDNAVLRTPVELVGENMKTVA
EFTVKEGERVPFSLAYAASHLRLPPARDPHTALARTENHWLEWSARGSIQGRWAEPIRRS
LITLRALAYEPTGGIVAAPTTSLPEQLGGTRNWDYRYCWLRDATITLLALMRGGYYDEAR
AWRSWLGRVTAGAPDQLQIMYGLAGERRLPEFEIDWLPGYQGAKPVRIGNNAVGQLQLDV
YGEVMNALHLARVGGLQADDTAWNVQCALLRHLTTIWQQPDEGIWETRGGRQHFTFSKVM
AWVAFDRALASAEMFKLEGPLDEWRATRAQIHAEVCEKAWNPSRNAFVQAYGSDQLDASV
LLMPLVSFLPPNDPRIKGTVEAIERDLMHDGFVMRYRTTEFDDGLPPGEGTFLACSFWMV
DNLALQGRLDEAIAMYERLLGLCNDVGLLAEEYDPAAKRLVGNFPQAFSHVSLVNTGLNL
MKHEQAMARATGQPSHNGMHQAELEAAASPDSGTATSPVA