Protein Info for H281DRAFT_00197 in Paraburkholderia bryophila 376MFSha3.1
Annotation: Phosphoenolpyruvate carboxylase, type 1
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 93% identical to CAPP_PARXL: Phosphoenolpyruvate carboxylase (ppc) from Paraburkholderia xenovorans (strain LB400)
KEGG orthology group: K01595, phosphoenolpyruvate carboxylase [EC: 4.1.1.31] (inferred from 88% identity to bpy:Bphyt_1068)Predicted SEED Role
"Phosphoenolpyruvate carboxylase (EC 4.1.1.31)" in subsystem Fermentations: Mixed acid or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.31)
MetaCyc Pathways
- mixed acid fermentation (13/16 steps found)
- ethene biosynthesis V (engineered) (19/25 steps found)
- formaldehyde assimilation I (serine pathway) (10/13 steps found)
- CO2 fixation into oxaloacetate (anaplerotic) (2/2 steps found)
- nitrogen remobilization from senescing leaves (6/8 steps found)
- partial TCA cycle (obligate autotrophs) (6/8 steps found)
- C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
- C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (11/18 steps found)
- C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (24/56 steps found)
KEGG Metabolic Maps
- Carbon fixation in photosynthetic organisms
- Pyruvate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.1.1.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1069 amino acids)
>H281DRAFT_00197 Phosphoenolpyruvate carboxylase, type 1 (Paraburkholderia bryophila 376MFSha3.1) VTSSGSARPARRNTASPNASTVDADASTASASSSAGAAQPGKAKRAPSSAKAANAAKAIK ADKTTVPAKPEKAVKASKADKPAKTASATSKAEVKTNSKAQAKNKAEDKAENKAPNLQAV SGDTVVAAPKTNGRTRADKDHPLFQDIRYLGRLLGDVLREQEGDAVFDVVETIRQTAVRF RREDDNAAVQTLDKKLRSLSPEQTVSVVRAFSYFSHLANIAEDRHRNRRHRIHALAGSAA QPGTIAYALERLVEAGAAATPVLQQFFNDALIVPVLTAHPTEVQRKSILDAEHDVARLLA ERDQQLTDRERAHNEAMLRARVTSLWQTRMLRDSRLTVADEIENALSYYRATFLEEIPAL YTDIEEALKEHGLEARLPPFFQMGSWIGGDRDGNPNVTAETLEHAIARQAEVIFEHYLEQ VHKLGAELSVSNLLAGASDELKALAEISPDRSPHRTDEPYRRALIGMYTRLAASARVRLG EGSVPLRSAGRGAAPIRATPYDDASEFVRDLHVLIDSLAAHHGAPLAAPRLAPLARAAEV FGFHLASIDLRQSSDIHEAVIAELLKRAGVHDDYAALAESEKLEVLLAELAQPRPLRLPY AEYSDLVKSELGVLEQARVTREKFGARAVRNYIISHTETVSDLVEVMLLQKETGLLQGRL GDANDPARAALMVIPLFETIPDLRNAPHIMRDLLALPGVDSIIEHQGNEQEVMLGYSDSN KDGGFLTSNWELYRAELALVSLFNERGITLRLFHGRGGTVGRGGGPTYQAILSQPPGTVD GQIRLTEQGEVIASKFGNPEIGRRNLETVVAATLEASLLPHGTAPAELPVFESTMQQLSD AAMASYRALVYETPGFKEYFFESTPISEIAELNIGSRPASRKLQDPKQRKIEDLRAIPWG FSWGQCRLLLTGWYGFGTAVAAYLDDAPSDAERGRRLAQLKKMHKTWPFFSTLLSNMDMV LAKTDLAVASRYAALVTDKKLRKHVFERIVAEWERTSKVLSEITGKSERLAENPLLARSI KNRFPYLDPLNHLQVELLKRYRAGDTNARVRRGIHLSINGIAAGLRNTG