Protein Info for H281DRAFT_00196 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Ubiquinone/menaquinone biosynthesis C-methylase UbiE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 PF07021: MetW" amino acids 28 to 133 (106 residues), 24.2 bits, see alignment E=1.3e-08 PF05175: MTS" amino acids 32 to 144 (113 residues), 28.4 bits, see alignment E=6.7e-10 PF01209: Ubie_methyltran" amino acids 37 to 156 (120 residues), 66.4 bits, see alignment E=1.4e-21 PF03848: TehB" amino acids 38 to 143 (106 residues), 23.1 bits, see alignment E=2.5e-08 PF13489: Methyltransf_23" amino acids 40 to 187 (148 residues), 62.5 bits, see alignment E=2.2e-20 PF13847: Methyltransf_31" amino acids 41 to 146 (106 residues), 74.2 bits, see alignment E=5.6e-24 PF00891: Methyltransf_2" amino acids 43 to 187 (145 residues), 23 bits, see alignment E=2.5e-08 PF03141: Methyltransf_29" amino acids 43 to 139 (97 residues), 25.6 bits, see alignment E=2.5e-09 PF13649: Methyltransf_25" amino acids 45 to 138 (94 residues), 83.2 bits, see alignment E=1e-26 PF08241: Methyltransf_11" amino acids 46 to 142 (97 residues), 91.5 bits, see alignment E=2.3e-29 PF08242: Methyltransf_12" amino acids 46 to 140 (95 residues), 57.4 bits, see alignment E=1.1e-18

Best Hits

Swiss-Prot: 35% identical to YCGJ_BACSU: Uncharacterized methyltransferase YcgJ (ycgJ) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 91% identity to bgf:BC1003_0916)

Predicted SEED Role

"SAM-dependent methyltransferase YafE (UbiE paralog)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (250 amino acids)

>H281DRAFT_00196 Ubiquinone/menaquinone biosynthesis C-methylase UbiE (Paraburkholderia bryophila 376MFSha3.1)
MKHHDQVADAFGSTAAAYLTSPTHATGADLRTLAESIAATPDATVLDMGCGAGHASFAVA
PHAKEVVAYDIAAPMLATVEGAAKDRGLANIRTQQGAAEKLPFADHAFDWVISRMSAHHW
HDVAPALAEVRRVLKPGGRVLFIDIAGIDHPLLDTHIQAIELLRDGSHIRDYRADEWIAL
FEAAGFKASIRERWRIDIEFSSWVSRMRTPEERVVAIQSLWRNSPDEVRHYFDVKEDGSF
KLDALMVEAA