Protein Info for H281DRAFT_00085 in Paraburkholderia bryophila 376MFSha3.1

Annotation: urease. Metallo peptidase. MEROPS family M38

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00449: Urease_alpha
  • amino acids 4 to 120 (117 residues), 189.8 bits, E=2.8e-60
• TIGR01792: urease, alpha subunit
  • amino acids 5 to 568 (564 residues), 996.8 bits, E=1.6e-304
• PF01979: Amidohydro_1
  • amino acids 126 to 454 (329 residues), 286.4 bits, E=5.2e-89

Best Hits

Swiss-Prot: 93% identical to URE1_BURCJ: Urease subunit alpha (ureC) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K01428, urease subunit alpha [EC: 3.5.1.5] (inferred from 99% identity to bgf:BC1003_0809)

MetaCyc: 75% identical to urease alpha subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease alpha subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

• urea degradation II (1/1 steps found)
• superpathway of allantoin degradation in plants (4/8 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Purine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

See A0A2Z5M8X8 at UniProt or InterPro

Protein Sequence (568 amino acids)

>H281DRAFT_00085 urease. Metallo peptidase. MEROPS family M38 (Paraburkholderia bryophila 376MFSha3.1)
MTLRIGRRAYAEMFGPTTGDRVRLADTELLIEVERDFTTYGEEVKFGGGKVIRDGMGQSQ
RVHADVVDTVVTNAVILDHWGIVKADIGIKNGRIAAIGKAGNPDIQPNVTIAIGASTEVI
AGEGLIVTAGGIDTHIHFISPQQIEEALASGVTTMLGGGTGPATGTNATTCTPGPWHLER
MLQAADGYPMNLGFLGKGNVSQPEPALEQIAAGAIGLKLHEDWGTTPAAIDNCLSVADDT
DTQVAIHTDTLNEAGFVEATVAAFKGRTIHTYHTEGAGGGHAPDIIKVCGEANVLPSSTN
PTRPYTVNTLEEHLDMLMVCHHLDPSIAEDIAFAESRIRRETIAAEDILHDLGALSMLSS
DSQAMGRVGEVIIRTWQTAHKMKVQRGALPEDTARHDNFRAKRYVAKYTINPAITHGIAH
EVGSIEPGKWADLVFWEPAFFGIKPSLILKGGMIAMAQMGDPNASIPTPQPVHYREMFAT
RGGALGRTSLTFVSQLAADAGVAERYGLNKRIVAVKNCRNITKADMIHNAWRPSISVDPE
TYQVIADGQLLTCEPATVLPMAQRYFLF