Protein Info for Ga0059261_3199 in Sphingomonas koreensis DSMZ 15582

Annotation: DNA topoisomerase IV, B subunit, proteobacterial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 659 TIGR01055: DNA topoisomerase IV, B subunit" amino acids 16 to 649 (634 residues), 744.7 bits, see alignment E=4e-228 PF02518: HATPase_c" amino acids 42 to 141 (100 residues), 56.5 bits, see alignment E=7.5e-19 PF00204: DNA_gyraseB" amino acids 239 to 408 (170 residues), 138.5 bits, see alignment E=3.4e-44 PF01751: Toprim" amino acids 438 to 548 (111 residues), 61.7 bits, see alignment E=1.2e-20 PF00986: DNA_gyraseB_C" amino acids 581 to 646 (66 residues), 77.5 bits, see alignment E=1.4e-25

Best Hits

Swiss-Prot: 61% identical to PARE_BARBK: DNA topoisomerase 4 subunit B (parE) from Bartonella bacilliformis (strain ATCC 35685 / NCTC 12138 / KC583)

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 82% identity to sch:Sphch_0383)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2M8WG19 at UniProt or InterPro

Protein Sequence (659 amino acids)

>Ga0059261_3199 DNA topoisomerase IV, B subunit, proteobacterial (Sphingomonas koreensis DSMZ 15582)
MSSDLFQPSSATPANSYDASSIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVLDNAMD
EAVAGHANRIEVTLDVGNRLTITDNGRGFPIDPHPKFPDKSALEVILSMLHSGGKFSGKA
YATSGGLHGVGISVVNALSVDTVVEVARDKQLYRQRFSRGQTLGPLEHLGGTPNRRGSLV
SFTPDSEIFGEMNFKPARLYKLARSKAYLFAGVEIRWKCAPELISDDTPAEAVFQFPGGL
ADHLKEQLGTREGATAEFFSGTQEFSDSQGRVEWAVAWPLWSDGSYSWYCNTIPTPDGGT
HEQGLRQALVRGIRAFGELVGQKKAKDITADDIMVGSELMLSVFIREPQFQSQTKDRLTS
PEATALVEKAVRDHFDHFLADRMDRGKALLGYVLDRMDERLRRKQEREVKRKTATSGRKL
RLPGKLTDCSADDPAGTELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASATSAKI
LANQEIADLIQALGCGTRKDCVVDNLRYERIVIMTDADVDGAHIATLLMTFFFQEMPDIV
RRGHLYLAQPPLYRLTAGTKSLYARDDAHRAEIEANAFKGRKVDVARFKGLGEMNPGQLR
ETTMDPKTRSMIRITLPQEYEERAGVKDLVDRLMGTNPAHRFAFIQENAARLDEEAIDA