Protein Info for Ga0059261_2636 in Sphingomonas koreensis DSMZ 15582

Annotation: 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 68% identical to UGL_BURM1: Ureidoglycolate lyase (Bmul_3283) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: None (inferred from 76% identity to hba:Hbal_2688)

MetaCyc: 64% identical to 5-hydroxy-2,4-dioxopentanoate hydrolase (Herbaspirillum huttiense)
RXN-12096 [EC: 3.7.1.26]; 3.7.1.26 [EC: 3.7.1.26]

Predicted SEED Role

"2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-) / 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic amino acid degradation (EC 4.1.1.68, EC 5.3.3.-)

MetaCyc Pathways

L-rhamnose degradation III (6/7 steps found)
D-xylose degradation VI (4/5 steps found)
D-arabinose degradation IV (3/6 steps found)
L-fucose degradation III (4/8 steps found)
L-arabinose degradation V (2/6 steps found)
4-hydroxyphenylacetate degradation (3/8 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.26 or 4.1.1.68 or 5.3.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2M8WEH9 at UniProt or InterPro

Protein Sequence (284 amino acids)

>Ga0059261_2636 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) (Sphingomonas koreensis DSMZ 15582)
MKLCRYGAPGAEKPGLIDADGRIRDLSNYVADIDAQTLSKETLAKLAGVDPATLKLVEGE
VRYGPCVTGTRQFVAIGLNYADHAAESNLPIPEEPVVFNKWVSCIQGPNDPVTIPKDSKK
TDWEVELGIVIGTAAHNVSEADALDHVAGYCVVNDVSERHWQTERGMTWDKGKGFPTFGP
IGPWMVTADEVGDPQNLSMWLSVNGKKVQDGNTRTMIFTVAQIVSYLSQIMTLLPGDVIT
TGTPPGVGLGQKPEPWYLKAGDVVELGIEKLGEQRQDFVAWQAK