Protein Info for Ga0059261_2527 in Sphingomonas koreensis DSMZ 15582

Annotation: 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 121 (116 residues), 129.9 bits, E=5.8e-42
• PF02882: THF_DHG_CYH_C
  • amino acids 124 to 280 (157 residues), 224.1 bits, E=5.7e-71

Best Hits

Swiss-Prot: 75% identical to FOLD_NOVAD: Bifunctional protein FolD (folD) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 75% identity to nar:Saro_3300)

MetaCyc: 57% identical to methenylTHF cyclohydrolase/methyleneTHF dehydrogenase subunit (Moorella thermoacetica)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• folate transformations II (plants) (8/11 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• L-histidine degradation III (4/6 steps found)
• folate transformations I (8/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• purine nucleobases degradation II (anaerobic) (11/24 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Sequence Analysis Tools

See A0A2M8WE59 at UniProt or InterPro

Protein Sequence (291 amino acids)

>Ga0059261_2527 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase (Sphingomonas koreensis DSMZ 15582)
MTAEIIDGKAFAAGLRARVGDAAAAFEAQAGRKAGLAVVLVGEDPASAVYVRSKGKATLA
ANMASFEHRLPADTGQDSLIALVDRLNADPAVDGILVQLPLPAHLDEQEVLLRIDPDKDV
DGFHPVNAGRLATGMEGFVPCTPLGCLMLLKDQLGDLSGKDAVVIGRSNIVGKPMAALLT
AASATVTLAHSRTKDLPGHVSRADIVVAAVGRAGFVKGEWLKPGATVIDVGINRTDNGLT
GDVEFDSAASVAGAITPVPGGVGPMTIACLLRNTLVAAHRREGLQLDQAAL