Protein Info for Ga0059261_0940 in Sphingomonas koreensis DSMZ 15582
Annotation: glutamyl-tRNA synthetase, bacterial family
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 69% identical to SYE2_NOVAD: Glutamate--tRNA ligase 2 (gltX2) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)
KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 69% identity to nar:Saro_2031)Predicted SEED Role
"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)
MetaCyc Pathways
- tRNA charging (19/21 steps found)
- superpathway of heme b biosynthesis from glutamate (7/10 steps found)
- tetrapyrrole biosynthesis I (from glutamate) (4/6 steps found)
- (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
- adenosylcobalamin biosynthesis II (aerobic) (18/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (17/36 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.1.1.17
Use Curated BLAST to search for 6.1.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2M8W9T8 at UniProt or InterPro
Protein Sequence (496 amino acids)
>Ga0059261_0940 glutamyl-tRNA synthetase, bacterial family (Sphingomonas koreensis DSMZ 15582) VLGALAAELAIDHSFWEQARLGATSEPATGTQIVTRFAPSPTGFLHIGGARTALFNWLFA RHHGGKFLLRIEDTDRARSTDAAIEAILDGMRWLGLDWDGDEVYQFARADRHAEVAHAML ESGHAYRCWMSQEEIAAQREEAQAAKKPYRIRSPWRDRTDGPLDQPHVVRIRAPREGATT IRDAVQGEVTVQNAELDDMILLRSDGTPTYMLAVVVDDHDMGVTHVIRGDDHLNNAFRQL LIIHAMDWAEPVYAHIPLIHGADGAKLSKRHGALGVDAYRDELGVLPEALFNHLLRLGWG HGDDEIIDRAQAVEWFDLSGVGKSPSRFDLKKLDSLNGHYIRAADDARLAQLAAPFLPFE ASPQQIDLLQRSMHALKPRAANLLEIADGSAFLFRTRPLEMDADAQALLGDDAKAILHSL HTALDALADWNTEALETAVRQVAESGGVKLGAAAQPLRAALTGRRTSPGIFDVLVLLGRE ESLGRIADQMAAPSGS