Protein Info for Ga0059261_0559 in Sphingomonas koreensis DSMZ 15582

Annotation: The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1042 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 342 to 361 (20 residues), see Phobius details amino acids 368 to 390 (23 residues), see Phobius details amino acids 396 to 415 (20 residues), see Phobius details amino acids 440 to 462 (23 residues), see Phobius details amino acids 473 to 498 (26 residues), see Phobius details amino acids 539 to 559 (21 residues), see Phobius details amino acids 872 to 890 (19 residues), see Phobius details amino acids 897 to 917 (21 residues), see Phobius details amino acids 923 to 946 (24 residues), see Phobius details amino acids 972 to 992 (21 residues), see Phobius details amino acids 999 to 1026 (28 residues), see Phobius details PF00873: ACR_tran" amino acids 3 to 1026 (1024 residues), 1222.4 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 4 to 1040 (1037 residues), 1534 bits, see alignment E=0

Best Hits

Swiss-Prot: 57% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 59% identity to aex:Astex_3378)

MetaCyc: 54% identical to multidrug efflux pump RND permease AcrD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-360

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2M8W8R8 at UniProt or InterPro

Protein Sequence (1042 amino acids)

>Ga0059261_0559 The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family (Sphingomonas koreensis DSMZ 15582)
MGISRFFIDRPIFAWVISIVIMLAGLLGLASLPVAQYPDVAPPTIRVSANYPGASAQTLE
TSVTQVIEQQLTGIDGLLYFQSSSDSSGSASITATFAKGTDPDTAQVQVQNKVQQATSRL
PSAVTSQGVTVTKANNDFLMIVALYDGSDKSTASDVADYLVTNFQDPLGRVEGVGQTQVF
GAQYAMRIWLDPNKLAARKLMPADVSAAVRAQNADVSAGQIGGLPAVKGQMLNAVVRGKS
RLTSVDQFNDIVVKTESDGSVVRLADVARVELGQESYAASAKLNGHPASGIAIQLATGAD
ALKTAAAVKERVAELSKDLPAGYAITYPRDTTDFVKLSIKEVIKTLIEAVVLVVIVMFVF
LQSWRATLIPAIAVPVVLLGTFGVLAVAGYSVNTLTLFAMVLAIGLLVDDAIVVVENVER
VMAEEGLNPREATIKSMSEIGSALVGIALVLSAVFLPMAFFGGSAGVIYRQFSVTIVSAM
VLSVVVALVLSPALTATLLKPGHHDPLERKGLAGRFNRWFARITGRYQDGAHSVIRRRLM
HFGVFIAITVVLGLLFARLPTSFLPEEDQGTAMVMYTLPAGATAERTEQVRAEIQRFYGN
EKDIANAFTVSGFSFGGSGQNAGMGFASLKPFDERPGAEHSAGAVNGKAMAAFSKIRDAQ
VFAMTPPPISGLGQSNGFTFQLLNSSGMPQAEFVALRDKLIAAAAKDPELMAVRASTLPD
TPQLDIKLDEAKLAVLGLSVDDVTSALSSAWGGTYINDFVDRGRVKRVYMQGDAPFRMLP
EDINQWQVRNPGTGEMVPFSSFATLSWEKGPNSVARFNGLPSYEIQGSAAAGTSSGEAMQ
RMVELQQELAPGTSYAWAGLSFQESQSSGQAPLLYGASILIVFLCLAALYESWSVPIAVL
LVLPLGIIGAVLAVTMRGLNNDIYFQVGLITTIGLAAKNAILIVEFAEDARRRGKDLVES
ALEAARLRLRPILMTSLAFIFGVLPLAIATGAGANSRIAIGTAVIGGMLTATVLAIFYVP
MFFVVIAKLFHSRAPKAQETQS